南方医科大学学报2017，Vol.37Issue(7)：957-961,5.DOI:10.3969/j.issn.1673-4254.2017.07.19
甘草酸对MRL/lpr小鼠狼疮性肾炎的治疗作用
Protective effects of glycyrrhizic acid against lupus nephritis in MRL/lpr mice
摘要
Abstract
Objective To investigate protective effect of glycyrrhizic acid (GA) against lupus nephritis in MRL/lpr mice and explore its underlying mechanisms. Methods Forty MRL/lpr mice were randomized equally into blank control group, dexamethasone (1.5 mg/kg) group, GA (20 mg/kg) group, and GA (40 mg/kg) group with corresponding treatments for 7 days, with 10 wild-type mice as the control group. Serum levels of uric acid and creatinine and inflammatory cytokines in the serum and kidney were tested after the treatments using enzyme-linked immunosorbent assays (ELISA). The pathological changes in the kidneys were detected using HE staining, and the protein expressions of NLRP3, ASC, caspase-1, IL-1β, p-NF-κB, NF-κB, p-IκBα, and IκBα were detected with Western blotting. Results GA obviously decreased serum levels of uric acid and creatinine, decreased inflammatory cytokines in the serum and kidney, ameliorated renal pathologies and inhibited the expressions of NLRP3, ASC, caspase-1, IL-1β, p-NF-κB, and p-IκBαproteins in MRL/lpr mice. Conclusion GA has protective effects against lupus nephritis in MRL/lpr mice.关键词
系统性红斑狼疮/甘草酸/肾脏/炎症Key words
systemic lupus erythematosus/glycyrrhizinic acid/kidney/inflammation引用本文复制引用
王元元,谢长好,李洪涛,陆杨,贾孝云,李杨磊,陈升,柴继侠,张佳佳,刘东..甘草酸对MRL/lpr小鼠狼疮性肾炎的治疗作用[J].南方医科大学学报,2017,37(7):957-961,5.基金项目
安徽省自然科学基金(1608085MH215) （1608085MH215）
安徽省高等学校省级自然科学研究重点项目(KJ2016A475) （KJ2016A475）
安徽省高等学校省级自然科学研究项目(KJ2015B009by) （KJ2015B009by）
蚌埠医学院科技发展基金(Bykf13A28) （Bykf13A28）
安徽省大学生创新创业项目(201610367028) （201610367028）
安徽省蚌埠医学院研究生科技创新项目(Byycx1609) （Byycx1609）
