南方医科大学学报2017，Vol.37Issue(7)：983-987,5.DOI:10.3969/j.issn.1673-4254.2017.07.23
FasL-844T/C基因多态性与退变腰椎间盘髓核FasL表达的关系
Association of FasL-844T/C gene polymorphism with FasL expression in the nucleus pulposus of degenerative lumbar intervertebral discs
摘要
Abstract
Objective To investigate the association of FasL-844T/C gene polymorphism with the magnetic resonance imaging (MRI) findings and FasL expression in the nucleus pulposus of degenerative lumbar intervertebral discs. Methods Lumbar MRI data, venous blood and nucleus pulposus were collected from 105 patients with lumbar disc herniation. The genotypes of FasL-844T/C gene of the patients were determined using matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Immunohistochemistry was used to detect the expression of FasL in the nucleus pulposus of the degenerative lumbar intervertebral discs. Results Compared with CC genotype, TT genotype of FasL-844T/C gene was associated with a significantly increased score of lumbar disc degeneration (P=0.003) as observed in MRI scan. FasL expression in the nucleus pulposus differed significantly between patients of FASL-844CC genotype and those of FASL-844TT genotype (P=0.048), but not between those of FASL-844CC and FASL-844CT genotypes (P=0.264). No significant association was found between MRI findings and FasL expression in the nucleus pulposus of the lumbar intervertebral discs. Conclusion FasL-844T/C gene polymorphism is correlated with the expression of FasL in the nucleus pulposus of the intervertebral disc in patients with lumbar disc herniation. MRI findings of the lumbar intervertebral discs do not correlate with the expression of FasL in the nucleus pulposus of the intervertebral discs.关键词
腰椎间盘突出症/腰椎间盘退变/髓核/FasL-844T/C基因多态性Key words
lumbar disc herniation/lumbar disc degeneration/nucleus pulposus/FASL-844T/C/gene polymorphisms引用本文复制引用
孙正明,彭毛加措,徐洪海,常彦海,吴学元,董向辉,凌鸣..FasL-844T/C基因多态性与退变腰椎间盘髓核FasL表达的关系[J].南方医科大学学报,2017,37(7):983-987,5.基金项目
陕西省"13115"科技创新工程计划(2015KTCL03-02) （2015KTCL03-02）
陕西省卫生厅科研基金(22012.D84) （22012.D84）
