南方医科大学学报2017，Vol.37Issue(8)：1028-1034,7.DOI:10.3969/j.issn.1673-4254.2017.08.05
内吗啡肽-1后处理的心肌保护作用及其对Erk1/2信号通路的影响
Effect of endomorphin-1 postconditioning against myocardial ischemia-reperfusion injury in rats and the role of Erk1/2 signaling pathway
摘要
Abstract
Objective To investigate the effect of postischemic treatment with endomorphin-1 (EM-1) against myocardial ischemia/reperfusion (IR) injury in rats and on extracellular signal regulated kinase 1/2 (Erk1/2)-dependent signaling pathway. Methods Sprague-Dawley rats were randomly divided into 5 groups, namely the sham-operated group, IR group, EM-1 post-treatment group (EM50 group), EM-1 post-treatment group with PD98059 treatment (EM50+PD group), and PD98059 post-treatment group (PD group). The hemodynamic indexes of the rats were recorded. After reperfusion, CK-MB, LDH, CTnI, MDA, IL-6, TNF-α, and SOD activities or contents were measured, the infarct size was determined, and the expression levels of Erk1/2, P-Erk1/2 and cleaved caspase-3 were detected using Western blotting. Results Compared with the sham group, the IR group showed significantly decreased heart rate and mean arterial pressure (P<0.05), which were increased obviously by EM-1 post-treatment (P<0.05). EM-1 post-treatment also resulted in significantly decreased LDH, CK-MB, CTnI, MDA, IL-6, and TNF-αactivities or contents (P<0.05), increased SOD activity (P<0.05), reduced the infarct size (P<0.05), and increased the expression level of P-Erk protein (P<0.05). Compared with EM50 group, EM50+PD group showed significantly decreased heart rate and mean arterial pressure (P<0.05), increased LDH, CK-MB, CTnI, MDA, IL-6, and TNF-αactivities or contents (P<0.05), decreased SOD activity, increased infarct size (P<0.05), and lowered expression of P-Erk protein (P<0.05). Conclusion Postischemic treatment with EM-1 protects the heart against IR injury by improving the cardiac function, inhibiting inflammation, and inhibiting oxidative stress and myocardial apoptosis, and Erk1/2 signaling pathway may be involved in this process.关键词
细胞外信号调节激酶1/2/心肌缺血后处理/心肌保护/内吗啡肽Key words
endomorphin-1/ischemia postconditioning/cardioprotection引用本文复制引用
王娅,刘明珠,李红俊,张蔚屏,高琴,李正红..内吗啡肽-1后处理的心肌保护作用及其对Erk1/2信号通路的影响[J].南方医科大学学报,2017,37(8):1028-1034,7.基金项目
国家自然科学基金(81472656) （81472656）
安徽省自然科学基金(1508085MH170) （1508085MH170）
蚌埠医学院研究生科研创新项目(Byycx1504) Supported by National Natural Science Foundation of China (81472656). （Byycx1504）
