南方医科大学学报2017，Vol.37Issue(8)：1047-1053,7.DOI:10.3969/j.issn.1673-4254.2017.08.08
益气活血方基于miR-124调控Wnt通路促进神经再生
Yiqihuoxue prescription promotes nerve regeneration by miR-124-mediated regulation of Wnt signaling in rats
摘要
Abstract
Objective To investigate the effect of Yiqihuoxue prescription (NLXT) on nerve regeneration in MCAO-R rat models of qi deficiency and blood stasis syndrome and explore the underlying mechanisms. Methods The rats were randomized into 4 groups, namely the control group, model group, NLXT group and TXL group. The rats in NLXT group and TXL group were treated with gavage of NLXT and TXL solutions, respectively. The NFDS, QDSS and BSSS of the rats were evaluated. The regional cerebral blood flow (rCBF) were dynamically monitored with laser Doppler scanning, and the volume of cerebral infarction was detected with TTC-dye;the expression levels of nestin and BrdU were assayed with immunohistochemistry and mmunofluorescent staining. The expressions of miRNA-124, Wnt3a, GSK3βandβ-catenin in the rat brain tissue were detected with PCR or Western blotting. Results NLXT and TXL both improved the neurological functions of the model rats, reduced NFDS, QDSS, and BSSS scores, decreased the volume of cerebral infarction, and promoted the recovery of the rCBF (P<0.01). Nestin and BrdU expression levels were significantly increased in the rat brain tissue in NLXT group and TXL group. NLXT significantly inhibited high expressions of miRNA-124 and Wnt3a in response to stress, and increased β-catenin expression level (P<0.01). NLXT and TXL produced no obvious effect on GSK3βexpression in the model rats (P>0.05). Conclusion NLXT can activate Wnt signaling by affecting miRNA-124 expression to offer neuroprotection and promote nerve regeneration in rats with cerebral ischemia with qi deficiency and blood stasis syndrome.关键词
气虚血瘀证/脑缺血/脑络欣通/神经再生/miRNA-124/Wnt通路Key words
qi deficiency and blood stasis syndrome/cerebral ischemia/Naoluoxintong/nerve regeneration/miRNA-124/Wnt signaling引用本文复制引用
谭辉,王键,尹婷婷,何玲,邓勇,李凤,王玉凤..益气活血方基于miR-124调控Wnt通路促进神经再生[J].南方医科大学学报,2017,37(8):1047-1053,7.基金项目
国家自然科学基金青年项目(81603523) （81603523）
安徽省高校自然科学研究重点项目(KJ2016A404) （KJ2016A404）
国家科技支撑计划(2012BAI26B00) Supported by National Natural Science Foundation of China (81603523). （2012BAI26B00）
