南方医科大学学报2017，Vol.37Issue(8)：1060-1065,6.DOI:10.3969/j.issn.1673-4254.2017.08.10
血清检测Glypican-3对原发性肝细胞癌诊断及疗效评估的临床意义
Value of detection of serum glypican-3 level in diagnosis and therapeutic effect evaluation of primary hepatocellular carcinoma
摘要
Abstract
Objective To explore the clinical value of detecting serum glypican-3 in the diagnosis and therapeutic effect evaluation of primary hepatocellular carcinoma (PHC). Methods Using sandwich ELISA, we detected serum glypican-3 levels in 60 patients with PHC, 60 with metastatic liver cancer, 50 with liver cirrhosis, 50 with chronic viral hepatitis, 20 with hepatic cyst, 20 with fatty liver, 20 with hepatic hemangioma and 20 with drug-induced hepatitis as well as in 40 healthy subjects (control). We also analyzed the changes in serum levels of glypican-3 and alpha fetoprotein (AFP) in PHC patients after treatment. Results PHC patients had significantly higher serum levels of glypican-3 than patients with other liver diseases and the control subjects (P<0.05). The levels of serum glypican-3 were significantly higher in patients with metastatic liver cancer, liver cirrhosis and viral hepatitis than in those with other benign liver diseases and the control subjects (P<0.05). Glypican-3 level was not associated with AFP level or liver function in PHC patients, in whom the positivity rates for glypican-3 and AFP were 65%and 56.7%, respectively. The detection rate of PHC increased to 85%by a combined detection of AFP and glypican-3. In the 23 PHC patients who responded positively to treatments, serum glypican-3 level showed a steady decline compared with that in 15 patients before treatment, while serum AFP level showed a similar decrease only in 10 patients. Conclusion Combined detection of glypican-3 and AFP is expected to improve the early diagnosis rate of PHC. The different thresholds of serum glypican-3 may play a role in the differential diagnosis of PHC and other various liver diseases. Glypican-3 may serve as a better marker than AFP with a high specificity and sensitivity for evaluating the therapeutic effect in PHC patients.关键词
原发性肝细胞肝癌/肿瘤标志物/磷酯酰肌醇蛋白聚糖-3/甲胎蛋白Key words
primary hepatocellular carcinoma/tumor markers/glypican-3/alpha fetoprotein引用本文复制引用
王媛媛,周陈杰,李静,周玲,李明松,肖冰..血清检测Glypican-3对原发性肝细胞癌诊断及疗效评估的临床意义[J].南方医科大学学报,2017,37(8):1060-1065,6.基金项目
国家消化系统疾病临床医学研究中心科技支撑项目(2015BAI13B07) （2015BAI13B07）
广州市科技计划项目(2014Y2-00088) （2014Y2-00088）
