南方医科大学学报2017，Vol.37Issue(8)：1104-1109,6.DOI:10.3969/j.issn.1673-4254.2017.08.17
巨噬细胞炎症蛋白-1β对人舌鳞癌细胞CAL-27增殖和凋亡的影响
Effect of macrophage inflammatory protein-1β on proliferation and apoptosis of human tongue squamous cell carcinoma CAL-27 cells in vitro
摘要
Abstract
Objective To detect CCR5 protein expression in different human tongue squamous cell carcinoma cells and observe the effect of macrophage inflammatory protein-1β (MIP-1β) on the proliferation and apoptosis of CAL-27 cells. Methods Western blotting and immunofluorescence staining were used to detect the expression of the CCR5, the receptor of MIP-1β, in 3 human tongue squamous cell carcinoma cells UM-1, CAL-27, and Tca-8113. CCK-8 assay was used to assess the proliferation of CAL-27 cells stimulated with 10, 20, and 40 ng/mL MIP-1β for 12, 24, or 48 h. The apoptosis of the cells stimulated with MIP-1β(10, 20, and 40 ng/mL) for 24 h was analyzed using flow cytometry with Annexin V/PI double staining. Results CCR5 expression was detected both on the membrane and in the cytoplasm in all the 3 tongue squamous cell carcinoma cell lines. At the concentrations of 10, 20, and 40 ng/mL, MIP-1β stimulation for 12 and 24 h significantly promoted the proliferation of CAL-27 cells (P<0.05);MIP-1βstimulation for 48 h at the concentrations 10 and 20 ng/mL, but not at 40 ng/mL, promoted the proliferation of CAL-27 cells (P<0.05). MIP-1βstimulation at 40 ng/mL for 24 produced the most obvious apoptosis-inducing effect in CAL-27 cells (P<0.05), while MIP-1β at 10 or 20 ng/mL did not induce obvious apoptosis in the cells (P>0.05). Conclusion CCR5 is expressed in all the 3 human tongue squamous cell carcinoma cells. MIP-1βcan promote the proliferation of CAL-27 cells but high concentrations of MIP-1βalso induced cell apoptosis. Prolonged stimulation of the cells with a high concentration of MIP-1βshows attenuated effect in promoting cell proliferation probably as a result of cell apoptosis induced by MIP-1β.关键词
舌癌/趋化因子/增殖/凋亡/巨噬细胞炎症蛋白-1βKey words
tongue cancer/chemokine/proliferation/apoptosis/macrophage inflammatory protein-1β引用本文复制引用
贾搏,邱小玲,褚洪星,孙翔,盘杰,王治平,赵建江..巨噬细胞炎症蛋白-1β对人舌鳞癌细胞CAL-27增殖和凋亡的影响[J].南方医科大学学报,2017,37(8):1104-1109,6.基金项目
国家自然科学基金(81670950) （81670950）
广州市科技计划项目(2014Y2-00109) （2014Y2-00109）
广东省科技计划项目(2014A020212549) Supported by National Natural Science Foundation of China (81670950). （2014A020212549）
