中国耳鼻咽喉头颈外科2017，Vol.24Issue(6)：320-324,5.DOI:10.16066/j.1672-7002.2017.06.012
变应性鼻炎嗅觉障碍大鼠嗅感受神经元的实验研究
Experimental research of the olfactory receptor neurons of olfactory dysfunction by allergic rhinitis
邵莉莉 1孙宝宾 1陶锋 1臧凤超 2蔡文君1
作者信息
- 1. 东南大学附属中大医院 耳鼻咽喉头颈外科,江苏 南京210009
- 2. 东南大学附属中大医院医学影像科,江苏 南京210009
- 折叠
摘要
Abstract
OBJECTIVE To set up an intranasal ovalbumin-induced animal model of allergic rhinitis(AR) accompanied with olfactory dysfunction in mice. By observing the olfactory pathway in mice using manganese-enhanced magnetic resonance imaging (MEMRI) and the relatively morphologic structural and immunological changes in olfactory epithelium, the influence of AR on olfactory receptor neurons(ORNs) was studied.METHODS Forty SD mice were randomly divided into two groups, the research group(n=30) and the control group(n=10). The research group was intraperitoneally injected and intranasal application of ovalbumin to establish an AR mice model. The olfactory function of the mice was evaluated by buried food test(BFT). ELISA was performed to measure the level of IgE in serum. MEMRI images were acquired with a 7.0 T micro-MR scanner. HE staining and immunohistochemistry were used to observe the tissues morphology change of olfactory mucosa and OMP expression.RESULTS The olfactory function evaluation of the AR mice model indicated that the incidence of olfactory dysfunction in AR mice was 40.0%. The AR mice with olfactory dysfunction had no signal enhancement in MEMRI. The olfactory epithelium became thinner, layer numbers of ORNs were decreased with disorder arrangement and the OMP expression was decreased in AR mice with olfactory dysfunction compared with that in AR mice without olfactory dysfunction(P=0.018) and the control group(P=0.0141).CONCLUSION An animal model of AR accompanied with olfactory dysfunction in mice was successfully established. The influence of AR on ORNs and thus cause the change of the olfactory pathway is one of the major pathogenesis of olfactory dysfunction in AR.关键词
鼻炎,变应性,常年性/鼻炎,变应性,季节性/嗅觉障碍/磁共振成像/嗅标记蛋白质/模型,动物Key words
Rhinitis,Allergic,Perennial/Rhinitis,Allergic,Seasonal/Olfaction Disorders/Magnetic Resonance Imaging/Olfactory Marker Protein/Models,Animal引用本文复制引用
邵莉莉,孙宝宾,陶锋,臧凤超,蔡文君..变应性鼻炎嗅觉障碍大鼠嗅感受神经元的实验研究[J].中国耳鼻咽喉头颈外科,2017,24(6):320-324,5.
