福建农业学报2017，Vol.32Issue(5)：503-507,5.DOI:10.19303/j.issn.1008-0384.2017.05.008
野生蕉ω-3脂肪酸去饱和酶基因FAD7密码子偏性分析
Codon Bias of ω-3 Fatty Acid Desaturase Gene (FAD7) in Musa itinerans
摘要
Abstract
The codon bias of ω-3 fatty acid desaturase gene, FAD7, of Musa itinerans was analyzed using CodonW, SPSS19.0, MEGA5.0, and the EMBOSS online program.It was also compared with the FAD7s from different plants and genomes of model organisms.The results showed the codon bias of FAD7 in M.itinerans to be low and leaning toward the synonymous codons with G or C.And, it appeared that the codons CUC, CAG and AGG received a high usage frequency in the gene.In comparison with the FAD7s of different plants, the components of its monocotyledons were bias toward the use G or C, while dicotyledons the opposite.A cluster analysis on the FAD7s based on codon usage bias and CDS showed a same result.Hence, the dicotyledons and monocotyledons in the gene could be satisfactorily differentiated.Based on the frequency of codon usage, the prokaryotic expression system seemed to be suitable for heterologous expression of the FAD7 in M.itinerans.关键词
野生蕉/密码子偏性/ω-3脂肪酸去饱和酶/聚类分析Key words
Musa itinerans/codon bias/ω-3 fatty acid desaturase/cluster analysis分类
农业科技引用本文复制引用
赖志宸,林玉玲..野生蕉ω-3脂肪酸去饱和酶基因FAD7密码子偏性分析[J].福建农业学报,2017,32(5):503-507,5.基金项目
福建省科技重大专项(2015NZ0002) （2015NZ0002）
国家香蕉产业技术体系建设专项(CARS-32-11) （CARS-32-11）
