法医学杂志2017，Vol.33Issue(3)：277-280,4.DOI:10.3969/j.issn.1004-5619.2017.03.013
SNaPshot技术应用于云南省部分人群ABO血型基因分型
Genotyping of ABO Blood Group in Partial Population of Yunnan Province by SNaPshot Technology
摘要
Abstract
Objective T o detect the genotype of A B O blood group by SN aPshot technology. Methods D N A w ere extracted from the peripheral blood sam ples w ith know n blood groups (obtained by serology) of 107 unrelated individuals in Y unnan. Six SN P loci of the 261th, 297th, 681th, 703th, 802th, and 803th nucleotide positions w ere detected by SN aPshot M ultiplex kit, and relevant genetics param eters w ere cal-culated. Results In 107 blood sam ples, the allele frequencies of types A , B , O A, and O G w ere 0.3551, 0.1682, 0.2300 and 0.2476, respectively, w hile that of types A G and cis A B w ere not detected. T he geno-typing results of A B O blood group w ere consistent w ith that of serologic testing. Conclusion SN aPshot technology can be adapted for genotyping of A B O blood group.关键词
法医遗传学/ABO血型系统/基因检测/SNaPshot技术Key words
forensic genetics/A B O blood group system/genetic testing/SN aPshot technology分类
社会科学引用本文复制引用
于书欣,曾发明,金岩章,万洪静,翟滇,邢豫明,程宝文..SNaPshot技术应用于云南省部分人群ABO血型基因分型[J].法医学杂志,2017,33(3):277-280,4.基金项目
云南省社会发展科技计划项目(2008GACA012-7) （2008GACA012-7）
