法医学杂志2017，Vol.33Issue(3)：281-283,3.DOI:10.3969/j.issn.1004-5619.2017.03.014
直接扩增法用于重大灾难事故中软骨的个体识别
Individual Identification of Cartilage by Direct Amplification in Mass Disasters
王传海 1徐程 1李湘秦 1吴勇 1杜舟1
作者信息
- 1. 深圳市公安局刑事警察支队刑事技术处,广东 深圳 518040
- 折叠
摘要
Abstract
Objective T o explore the effectiveness of direct am plification for the ST R analysis of carti-lage, and to accelerate the effectiveness of disaster victim identification. Methods E ighty-eight cartilage sam ples w ere directly am plified by Pow erPlex誖21 kit, and the results of genotyping w ere com pared w ith that obtained by the m agnetic beads m ethod. Results In 88 cartilage sam ples, the ST R genotypes w ere successfully detected from 84 sam ples by direct am plification and m agnetic beads m ethod, and both the results of genotyping by tw o m ethod w ere consistent. Conclusion D irect am plification w ith Pow er-Plex誖21 kit can be used for ST R genotyping of cartilages. T his m ethod is operated easily and prom ptly, w hich has a potential application in the individual identification of m ass disasters.关键词
法医遗传学/软骨/基因分型技术/直接扩增法/个体识别Key words
forensic genetics/cartilage/genotyping techniques/direct am plification/individual identification分类
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王传海,徐程,李湘秦,吴勇,杜舟..直接扩增法用于重大灾难事故中软骨的个体识别[J].法医学杂志,2017,33(3):281-283,3.
