法医学杂志2017，Vol.33Issue(3)：293-300,8.DOI:10.3969/j.issn.1004-5619.2017.03.017
DNA甲基化检测方法及其法医学应用研究进展
Research Progress on the Detection Method of DNA Methylation and Its Applica-tion in Forensic Science
聂燕钗 1俞丽娟 1管桦 1赵颖 1荣海博 1姜伯玮 1张涛1
作者信息
摘要
Abstract
A s an im portant part of epigenetic m arker, D N A m ethylation involves in the gene regulation and attracts a w ide spread attention in biological auxology, geratology and oncology fields. In forensic science, because of the relative stable, heritable, abundant, and age-related characteristics, D N A m ethyla-tion is considered to be a useful com plem ent to the classic genetic m arkers for age-prediction, tissue-identification, and m onozygotic tw ins' discrim ination. V arious m ethods for D N A m ethylation detection have been validated based on m ethylation sensitive restriction endonuclease, bisulfite m odification and m ethylation-C pG binding protein. In recent years, it is reported that the third generation sequencing m ethod can be used to detect D N A m ethylation. T his paper aim s to m ake a review on the detection m ethod of D N A m ethylation and its applications in forensic science.关键词
法医遗传学/DNA甲基化/综述/双生,单卵/三代测序Key words
forensic genetics/D N A m ethylation/review/tw ins/m onozygotic/third generation sequencing分类
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聂燕钗,俞丽娟,管桦,赵颖,荣海博,姜伯玮,张涛..DNA甲基化检测方法及其法医学应用研究进展[J].法医学杂志,2017,33(3):293-300,8.
