高等学校化学学报2017，Vol.38Issue(7)：1198-1202,5.DOI:10.7503/cjcu20160960
基于1,8-萘酰亚胺的半胱氨酸荧光探针的合成及在生物成像方面的应用
A Sensitive Fluorescent Probe for Cysteine Based on 1,8-Naphthalimide Derivative and Application in Bioimaging
摘要
Abstract
A turn-on fluorescent probe glyoxal mono(N-hydroxyethyl-1,8-naphthalimide-4-hydrazone)(NAD) was designed and synthesized for the detection of cysteine(Cys).The fluorescent probe shows high selectivity and sensitivity toward Cys over other various amino acids.When Cys was added to the solution of the probe,the C=N isomerization process was inhibited by an intramolecular hydrogen bond,resulting in enhanced fluorescence.Furthermore,the probe NAD could effectively detect Cys in living cells.关键词
荧光探针/半胱氨酸/细胞成像/1,8-萘酰亚胺衍生物Key words
Fluorescent probe/Cysteine/Cells imaging/1/8-Naphthalimide derivative分类
化学化工引用本文复制引用
杨敬敬,俞岳文,王炳祥,江玉亮..基于1,8-萘酰亚胺的半胱氨酸荧光探针的合成及在生物成像方面的应用[J].高等学校化学学报,2017,38(7):1198-1202,5.基金项目
国家自然科学基金(批准号:21202101)和江苏高校优势学科建设工程项目资助.Supported by the National Natural Science Foundation of China(No.21202101) and the Project Fund of the Priority Academic Program Development of Jiangsu Higher Education Institutions,China. （批准号:21202101）
