口腔疾病防治2017，Vol.25Issue(7)：414-419,6.DOI:10.12016/j.issn.2096-1456.2017.07.002
Notch信号及自噬在三氧化矿化聚合物促人牙髓细胞体外分化中的作用
Effect of Notch signaling and autophagy on MTA induced differentiation of human dental pulp cells in vitro
摘要
Abstract
Objective The aim of this study is to investigate the roles of Notch signaling and autophagy on mineral trioxide aggregate (MTA) induced differentiation of human dental pulp cells (hDPCs). Methods Third molars from healthy human were collected and hDPCs were isolated by a combined digestion of collagenase Ⅰand dispaseⅡ. Real time PCR were used to test the mRNA expression levels of alkaline phosphatase (ALP), runt-related transcription factor 2 (Runx2) and dentin sialophoprotein (DSPP) in MTA treated hDPCs in different time (24 h, 3 d and 7 d). The mineral-ization nodules formed by hDPCs with or without MTA treatment were detected by Von Kossa staining. Expressions of Notch1, Jagged1, Hes1, LC3Ⅱ/LC3Ⅰand p62 in wild type and MTA treated hDPCs were detected by western blotting. Results MTA extracted in a concentration of 0.1 mg/mL could promote the differentiation of hDPCs. Compared with that of wild type hDPCs, the expressions of Notch1, Hes1, or Jagged1 and p62 (P<0.01) in MTA treated hDPCs were significantly increased. MTA treatment showed inhibition effects on autophagy flux similar to Bafilomycin A 1, a specific inhibitor of fusion between autophagosomes and lysosomes. Conclusion MTA could promote hDPCs differentiation with highly relevant in stimulating Notch1-Jagged1-Hes1 signaling and inhibition of autophagy flux.关键词
三氧化矿化聚合物/牙髓细胞/分化/Notch/自噬Key words
Mineral trioxide aggregate/Dental pulp cells (DPCs)/Differentiation/Notch/Autophagy分类
医药卫生引用本文复制引用
何飞,郑雨燕,仇伟,张国权,麦穗..Notch信号及自噬在三氧化矿化聚合物促人牙髓细胞体外分化中的作用[J].口腔疾病防治,2017,25(7):414-419,6.基金项目
广东省自然科学基金项目(2014A030313068) （2014A030313068）
深圳市科创委科技计划项目(JCYJ201416122811975) （JCYJ201416122811975）
