口腔疾病防治2017，Vol.25Issue(8)：482-487,6.DOI:10.12016/j.issn.2096-1456.2017.08.002
正畸力作用下大鼠前扣带皮质层中信号转导与转录激活因子1的表达变化
Changes of signal transducer and activator of transcription 1 expression in the anterior cingulate cortex under orthodontic force in rats
摘要
Abstract
Objective To study the change of STAT1 expression in the anterior cingulate cortex on rats under orth-odontic force, and to further explore the roles of STAT1 and related JAK-STAT1 signaling pathway in the mediation and regulation of pain during tooth movement. Methods 112 male Sprague-Dawley (SD) rats (225±25 g) were used in this study. They were randomly divided into experimental groups (96 rats) and control groups (16 rats). All rats were in-stalled bilateral maxillary device for tooth movement models. Rats in the experimental groups were applied 80g orth-odontic force on both sides and were divided into six subgroup 4 h, 12 h, 24 h, 2 d, 3 d, 7 d, with 16 rabbits in each subgroup. The control groups were only installed the same orthodontic devices, without the application of orthodontic force. Brain tissue of the anterior cingulate cortex was isolated after 4 h, 12 h, 24 h, 2 d, 3 d, 7 d since experiment, and the expression level of STAT1 and p-STAT1 was analyzed with the method of immunofluorescence and PCR. Results For the immunofluorescence result, there was significant difference in STAT1 expression between control groups and dif-ferent experimental groups at different time points in total (P< 0.05). The STAT1 expression amount in the 4 h group decreased significantly when compared with the control group (P<0.05);to the 2 d group, the difference is still statisti-cally significant (P< 0.01). 3 d group, 7 d group and control group had no statistically significant difference. The STAT1 expression amount in 4 h group, 12 h group, 24 h group was significantly lower than that in 3 d and 7 d groups, differences were statistically significant (P<0.05). The STAT1 expression in the 2 d group was significantly lower than that of 7 d (42.35 ± 5.77) group, the difference was statistically significant (P< 0.05). There was significant difference in p-STAT1 expression between control groups and different experimental groups at different time points in total (F=623.518, P<0.05). The p-STAT1 expression amount in experimental groups were higher than that in the control group (P<0.05). The p-STAT1 expression in 4 h group was lower than that in 12 h and 24 h group and higher than that in 2 d, 3 d and 7 d groups, of which the differences were statistically significant (P < 0.05). The p-STAT1 expression in 12 h group was lower than that in 24 h group and higher than that in 2 d, 3 d and 7 d groups, of which the differences were statistically significant (P<0.05). For the PCR result, the expression of mRNA in STAT1 of experimental groups of 4 h, 12 h, 24 h, 2 d, 3 d, 7 d and the control groups were not statistically significant (P>0.05). Conclusions After apply-ing orthodontic force, the expression of STAT1 decreased transiently and the expression of p-STAT1 increased transient-ly. The reduction of STAT1 was probably caused by the phosphorylation of STAT1 and decrease in the translation level of STAT1, rather than changes in the transcriptional levels. The orthodontic pain might be related with the activation of STAT1 into phosphorylated STAT1.关键词
正畸力/疼痛/前扣带皮质层/信号转导与转录激活因子1/磷酸化信号转导与转录激活因子1Key words
Orthodontic force/Pain/ACC/STAT1/pSTAT1分类
医药卫生引用本文复制引用
郑翼,赵转浓,王艺羲,曹阳,刘楚峰..正畸力作用下大鼠前扣带皮质层中信号转导与转录激活因子1的表达变化[J].口腔疾病防治,2017,25(8):482-487,6.基金项目
国家自然科学基金(81170990) （81170990）
