口腔疾病防治2017，Vol.25Issue(8)：488-493,6.DOI:10.12016/j.issn.2096-1456.2017.08.003
低氧激活HIF-1α抑制人牙周膜成纤维细胞的成骨分化
Effects of hypoxic condition on osteogenic differentiation of human periodontal ligament cells via hypoxia in-ducible factor-1α
摘要
Abstract
Objective To investigate the effects of hypoxia on osteogenic differentiation of periodontal ligament cells (PDLCs) and the role of hypoxia inducible factor-1α(HIF-1α) in this process. Methods Human PDLCs were iso-lated and identified by checking the expression of vimentin and cytokeratin. PDLCs were cultured in normoxia (20%O2) or hypoxia (1% O2) for 12-72 h. Changes of alkaline phosphatase (ALP) activity and mRNA expressions of osteogenic markers ALP, collagen-I (COL1) and runt related transcription factor 2 (RUNX2) were detected. Western blot was used to detect the expression of HIF-1α. After transfected with HIF1α-siRNA, the expressions of HIF-1αand osteogenic dif-ferentiation markers were furthered detected. The statistics were analyzed with SPSS13.0. Results Positive vimentin but negative cytokeratin were observed in primary cultured PDLCs. ALP activity and mRNA expressions of ALP, COL1 and RUNX2 were decreased in PDLCs in hypoxia for 48 h, while HIF-1α expression was increased. After knocking down of HIF-1α with siRNA, HIF-1α was significantly reduced in PDLCs under hypoxia, while ALP activity and mRNA expressions of osteogenic markers were significantly increased. Conclusion Hypoxia may inhibit osteogenic differentiation of PDLCs via upregulated HIF-1α.关键词
牙周炎/人牙周膜成纤维细胞/低氧/成骨分化/低氧诱导因子-1αKey words
Periodontitis/Periodontal ligament cells/Hypoxia/Osteogenic differentiation/Hypoxia induc-ible factor-1α分类
医药卫生引用本文复制引用
庞静雯,吴亚霖,徐婷,庄秀妹..低氧激活HIF-1α抑制人牙周膜成纤维细胞的成骨分化[J].口腔疾病防治,2017,25(8):488-493,6.基金项目
国家自然科学基金青年基金(81600899) （81600899）
