口腔疾病防治2017，Vol.25Issue(8)：494-497,4.DOI:10.12016/j.issn.2096-1456.2017.08.004
氯化钙浓度对念珠菌溶血活性的影响
Influence of concentrations of CaCl2 on hemolytic activity of candida strains
摘要
Abstract
Objective To investigate the influence of different concentrations of CaCl2 on the hemolytic activity of candida strains. Methods A total of 25 candida strains were selected, including 21 candida strains recovered from clinical specimens, and 4 reference strains of C. albicans (ATCC 90028) (American Type Culture Collection, Manassas, VA, USA), C. glabrata (ATCC 90030), C. krusei (ATCC 6258), and C. tropicalis (ATCC 13803) respectively. CaCl2 was added at concentrations of 0%, 0.5%, 1%and 2.5 g%(wt/vol). Plates were cultured at 37℃in a 5%CO2 atmosphere for 24-72 h, and the hemolytic index (Hi) was analyzed. Results Comparsions of the hemolytic indices among the groups treated with 0.5%CaCl2 (Hi=2.247 ± 0.079), 1%CaCl2 (Hi=2.013 ± 0.092), 2.5%CaCl2 (Hi=2.150 ± 0.066) and the control (Hi=2.749 ± 0.103) reached statistical significance (P<0.001). Conclusion CaCl2 may produce a de-crease activity in the hemolysis of candida species.关键词
念珠菌/毒力因素/溶血活性/电解质/氯化钙Key words
Candida species/Virulence factors/Hemolytic activity/Electrolytes/CaCl2分类
医药卫生引用本文复制引用
万蕾,卢海宾,罗刚..氯化钙浓度对念珠菌溶血活性的影响[J].口腔疾病防治,2017,25(8):494-497,4.基金项目
广东省自然科学基金(2014A030310146) （2014A030310146）
广东省医学科学技术研究基金(A2016241). （A2016241）
