广东医学2017，Vol.38Issue(14)：2147-2150,4.
风湿性心脏瓣膜病合并心房颤动中胶原蛋白表达与分布
A study on expression and distribution of collagen in rheumatic heart disease with atrial fibrillation
摘要
Abstract
Objective To investigate the expression and distribution of collagens in patients with rheumatic valvular heart disease.Methods Thirty-three patients with rheumatic heart disease, who accepted valve surgery, were divided into sinus rhythm group and atrial fibrillation group.Right atrial appendage tissues were obtained during operation.Functional analysis of differentially expressed genes was performed by transcriptomics.By RT-PCR, we detected the expression of type I-IV collagen mRNA in myocardial tissue.The distribution of collagen in myocardium was observed by immunohistochemistry.Results Compared to sinus rhythm group, the left atrial diameter was significantly expanded.Transcriptomics showed that COL1A2, COL2A1, COL3A1 and COL4A3 interacted with COL5A2, COL6A3, COL11A1, and various cytokines.The results of RT-PCR showed that COL1A2, COL2A1, COL3A1, and COL4A3 were up-regulated in atrial fibrillation group.Furthermore, COL3A1 and COL4A3 were significantly up-regulated (P<0.05).Positive type Ⅰ collagen staining was predominantly on the basement membrane beneath the vascular endothelial and partially in the intercellular space.Myocardial collagen fiber was stained with type Ⅱ collagen.Type Ⅲ collagen was positive on capillary endothelial and vascular basement membrane.Type Ⅳ collagen was detected around myocyte, lipocyte, vascular smooth muscle and the vascular endothelial basement membrane.Conclusion Abnormal expression of COL3A1 and COL4A3 is revealed in atrial myocytes, interacting with various cytokines.It is related with the occurrence and development of atrial fibrillation.关键词
风湿性心脏瓣膜病/心房颤动/胶原纤维Key words
rheumatic heart disease/atrial fibrillation/collagen引用本文复制引用
周冬冬,姜亦瑶,袁超,施超..风湿性心脏瓣膜病合并心房颤动中胶原蛋白表达与分布[J].广东医学,2017,38(14):2147-2150,4.基金项目
天津市企业博士后创新项目择优资助计划(编号:2015-001),天津市滨海新区卫生局基金项目(编号:2014BHKY010),安徽省高校优秀青年人才支持计划重点项目(编号:gxyqZD2016167),安徽省学术和技术带头人及后备人选学术科研活动资助项目(编号:2015H070) （编号:2015-001）
