感染、炎症、修复2016，Vol.17Issue(3)：131-135,封2,6.DOI:10.3969/j.issn.1672-8521.2016.03.001
低浓度过氧化氢预处理骨髓间充质干细胞对创面愈合的促进作用
Effect of bone mesenchymal stem cells pre-incubated with low-concentration hydrogen peroxide on promoting wound healing
摘要
Abstract
Objective:To observe the survival of transplanted bone marrow mesenchymal stem cells (BMSCs) preincubated with low-concentration hydrogen peroxide (H2O2) in skin wounds of rats with acute skin defect,and to evaluate its efficiency for promoting wound healing.Methods:Seventy-five SD rats with acute full-thickness skin defect (round,1 cm in diameter) at the dorsal midline were randomly divided into three groups:normal saline (NS) group,BMSCs alone group and H2O2 pre-incubated BMSCs group,with 25 animals in each group.After the excisional wounds were performed,rats in the three groups were immediately injected with normal saline,BMSCs (l×l07),and BMSCs (1×107) pre-incubated by 50 μmol/L H2O2 for 12 hours,respectively.In order to trace the transplanted BMSCs,BMSCs were labeled with CM-Dil before transplantation.The wounds were photographed on day 0 to 15,the image was analyzed by Image Proplus 5.0 software,and the healing rate of the wound was calculated.The abundance of transplanted cells at the wound sites was observed under fluorescence microscope on days 1,2,and 5.Immunohistochemical staining of CD31 was used to evaluate the changes in microvessel density at the wound sites.Results:Compared to the BMSCs alone group,the abundance of transplanted cells at wound sites was significantly increased when BMSCs were pre-incubated by H2O2 before transplantation (P＜0.05 or P＜0.01).The numbers of BMSCs at the wound sites were significantly decreased on days 2 and 5 after injection of BMSCs compared to day 1 (P＜0.05) in BMSCs alone group,while the decrease was not statistically significant in the H2O2-preincubated BMSCs group (P＞0.05).The healing rate of wound in BMSCs alone group and H2O2-preincubated BMSCs group were significant higher than that in the NS group on day 5 to day 15 post-injury (P＜0.05 or P＜0.01),and it was higher in the H2O2-preincubated BMSCs group than in the BMSCs alone group on day 5 to day 10 post-injury (P＜0.05).The density of microvessels at wound sites showed no significant difference between the two BMSCs groups on day 3 post-injury.However,the microvessel density was significantly increased in H2O2-preincubated BMSCs group compared with the BMSCs simple group on days 5,7 and 10 post-injury (P＜0.05).Conclusions:A low-concentration H2O2 pre-incubation could prolong the survival time of implanted BMSCs,and promote angiogenesis and wound healing,thus providing a novel strategy to improve the efficacy of mesenchymal stem cells-based therapy for cutaneous wound.关键词
骨髓间充质干细胞/过氧化氢/预处理/移植/创面愈合Key words
Bone marrow mesenchymal stem cells/Hydrogen peroxide/Pre-incubation/Transplantation/Wound healing引用本文复制引用
梁东兰,黄宏,荆昭,徐祥,刘晓萍..低浓度过氧化氢预处理骨髓间充质干细胞对创面愈合的促进作用[J].感染、炎症、修复,2016,17(3):131-135,封2,6.基金项目
国家自然科学基金资助项目(81372059,81571912) （81372059,81571912）
国家重点基础研究发展规划项目(2012CB518105) （2012CB518105）
重庆市基础与前沿研究计划院士专项项目(cstc2014jcyjys10002) （cstc2014jcyjys10002）
