国际妇产科学杂志2017，Vol.44Issue(2)：163-166,后插1,5.
CXCR7沉默对HeLa细胞侵袭和迁移能力的影响及其在宫颈癌中的表达
The Effects of Inhibiting CXCR7 Gene Expression on Migration and Invasion of HeLa Cells and Expression of Cervical Cancer Tissues
摘要
Abstract
Objective:To investigate the effect of chemokine receptor 7 (CXCR7) silencing on invasion and migration of HeLa cells and its expression in cervical carcinoma and normal cervical tissues.Methods:Respectively,after the HeLa cells were transfected with plasmid expressing CXCR7 shRNA (CXCR7-shRNA) or negative control shRNA (Ctrl-shRNA),detecting the changes in cell migration and invasion of them by Transwell method.Immunohistochemistry was used to detect the expression of CXCR7 in cervical cancer tissues and non cancerous cervical tissues.Results:CXCR7 protein was positive in 16 cases of cervical cancer tissrues.Transwell invasion and migration assay showed that the number of CXCR7-shRNA transfected group was significantly lower than that of Ctrl-shRNA transfection group and non transfection group.Conclusions:The expression of CXCR7 was significantly higher in cervical cancer tissues,and the expression of CXCR7 could inhibit the invasion and migration of cervical cancer cells.Therefore,CXCR7 may be an important target for the prevention and treatment of cervical cancer.关键词
受体,趋化因子/HeLa细胞/肿瘤侵润/细胞运动Key words
Receptors,chemokine/Hela cells/Neoplasm invasiveness/Cell movement引用本文复制引用
王静雯,王思念,孙锁柱,张新,于楠,李峰生..CXCR7沉默对HeLa细胞侵袭和迁移能力的影响及其在宫颈癌中的表达[J].国际妇产科学杂志,2017,44(2):163-166,后插1,5.基金项目
辽宁省自然科学基金(2015020287) （2015020287）
