临床与病理杂志2017，Vol.37Issue(5)：881-888,8.DOI:10.3978/j.issn.2095-6959.2017.05.002
川芎嗪对肾间质纤维化中TGF-β1/miR-29表达的影响
Effect of tetramethylpyrazine on TGF-β 1/miR-29 expression in renal interstitial fibrosis
摘要
Abstract
Objective:To observe the effect of tetramethylpyrazine (TMP) on renal interstitial fibrosis,interstitial injury and TGF-β1/miR-29 expression in UUO model rats.Methods:Fifty male Wistar rats were randomly divided into five groups:normal control group (Ctrl),sham operation group (Sham),model group (UUO),valsartan group (VAN) and TMP group (TMP) with 10 in each group.After corresponding treatment,all rats were sacrificed and the left renal were used to evaluate pathology changes of rats' kidney tissue with HE and Masson staining;while using SP immunohistochemical method to detect the lesions in the expression of TGF-β 1;and finally compare the gene expression of TGF-β 1 and miR-29 in each group of by RT-PCR assay.Results:HE staining results showed that the interstitial injury scores of Ctrl,Sham,UUO,VAN and TMP group were 0.27±0.10,0.30±0.12,8.79± 1.03,6.23±0.81 and 4..57±0.74,respectively.The results of Masson staining showed that scores of Ctrl,Sham,UUO,VAN and TMP group were 0.21±0.03,0.23±0.06,2.49±0.33,1.63±0.07 and 1.32±0.04,respectively.SP immunohistochemical staining showed that compared with the control group,model group has a higher expression of TGF-β 1,while treatment with valsartan and TMP could reverse the up-regulation of TGF-β 1 and TMP is more effective than valsartan.RT-PCR results showed that TGF-β1 mRNA expression was increased,while miR-29 mRNA expression decreased in model group.VAN group and TMP group have a significantly lower expression of TGF-β 1 mRNA level compared to the model group,while miR-29 mRNA expression was significantly higher in both VAN group and TMP group;and TGF-β1 mRNA expression in the TMP group was higher than that in VAN group.Conclusion:Valsartan and TMP could significantly reduce the renal histological damage in renal interstitial fibrosis and TMP is more effective than valsartan.Furthermore,TMP could inhibit TGF-β 1 gene expression,while promoting the miR-29 expression.关键词
单侧输尿管结扎术/肾间质纤维化/川芎嗪/TGF-β1/miR-29Key words
unilateral ureteral obstruction/renal interstitial fibrosis/tetramethylpyrazine/TGF-β 1/miR-29引用本文复制引用
徐梅,孙升云..川芎嗪对肾间质纤维化中TGF-β1/miR-29表达的影响[J].临床与病理杂志,2017,37(5):881-888,8.基金项目
广东省中医药局科研项目(20171075) （20171075）
广东省科技计划项目(2014A020221015).This work was supported by Project of Administration of Traditional Chinese Medicine of Guangdong Province (20171075) and Science and Technology Planning Project of Guangdong Province (2014A020221015),China. （2014A020221015）
