临床与病理杂志2017，Vol.37Issue(5)：908-911,4.DOI:10.3978/j.issn.2095-6959.2017.05.006
基于热休克蛋白家族分析醇提蛇床子对红色毛癣茵抗真菌作用的机制
Mechanisms of the inhibition of Cnidium monnieri (L.) Cuss to Trichophyton rubrum based on heat shock protein
摘要
Abstract
Objective:To detect the effects and mechanisms of Cnidium monnieri (L.) Cuss in Trichophyton rubrum.Methods:Disk diffusion was used to determine the susceptibility and minimum inhibitory concentration (MIC) of Trichophyton rubrum.Real-time PCR and Western blot were used to determine the mRNA and protein levels of heat shock protein (Hsp) in Trichophyton rubrum after treated with Cnidium monnieri (L.) Cuss.Results:The proliferative ratio of Trichophyton rubrum was inhibited significantly by Cnidium monnieri (L.) Cuss.The levels of Hsp60 and Hsp90 mRNA and protein were lower in Trichophyton rubrum than the untreated ones.Conclusion:Cnidium monnieri (L.) Cuss could inhibit the proliferative ratio of Trichophyton rubrum by suppressing Hsp60 and Hsp90.关键词
醇提蛇床子/红色毛癣菌/最低抑菌浓度/热休克蛋白/增殖Key words
Cnidium monnieri (L.) Cuss/Trichophyton rubrum/minimum inhibitory concentration/heat shock protein/proliferation引用本文复制引用
王丽纳,朱焱,庄永灿,王红兰..基于热休克蛋白家族分析醇提蛇床子对红色毛癣茵抗真菌作用的机制[J].临床与病理杂志,2017,37(5):908-911,4.基金项目
福建省青年科研课题(2014-2-48).This work was supported by Project of Youth Scientific Research of Fujian Province,China (2014-2-48). （2014-2-48）
