国际病理科学与临床杂志2017，Vol.37Issue(5)：925-931,7.DOI:10.3978/j.issn.2095-6959.2017.05.009
沉默miR-21对子宫内膜癌顺铂耐药细胞株Ishikawa/DDP的影响
Effect of silencing miR-21 on Ishikawa/DDP cisplatin resistant cell in endometrial carcinoma
项锦红 1丁秋霞 1黄强 1杨鹰1
作者信息
- 1. 第三军医大学第二附属医院妇产科,重庆400000
- 折叠
摘要
Abstract
Objective:To observe the effects of silencing miR-21 on Ishikawa/DDP cisplatin resistant cell in endometrial carcinoma.Methods:Ishikawa/DDP cisplatin resistant cell was transfected with miR-21inhibitor by Lipofectamine 2000.At the same time,control group and drug resistant group were set up.The expression of miR-21,MDR1,Bax and Bcl-2 was detected by reverse transcription PCR.The protein expression of P-gp,Bax and Bcl-2 was detected by Western blot.The sensitivity of Ishikawa/DDP cells to cisplatin was detected by MTT.The cell apoptosis was detected by flow cytometry.Re sults:Compared with the drug resistant group and the control group,the mRNA expression of miR-21,MDR1,Bcl-2 in the inhibitor group significantly decreased (P＜0.01).The mRNA expression of Bax in the inhibitor group significantly increased (P＜0.001).The protein expression of P-gp,Bcl-2 in the inhibitor group significantly decreased (P＜0.05).The protein expression of Bax in the inhibitor group significantly increased (P＜0.001).TheICS0 value in the inhibitor group significantly decreased (P＜0.001).The apoptosis rate in the inhibitor group significantly increased (P＜0.001).Conclusion:Silencing miR-21 can significantly increase the sensitivity of Ishikawa/DDP cells to cisplatin and promote apoptosis.The specific mechanism may be related to down regulationof MDR1,P-gp,Bcl-2,as well as up regulation of Bax.关键词
miR-21/子宫内膜癌/顺铂/耐药Key words
miR-21/endometrial carcinoma/cisplatin/drug resistance引用本文复制引用
项锦红,丁秋霞,黄强,杨鹰..沉默miR-21对子宫内膜癌顺铂耐药细胞株Ishikawa/DDP的影响[J].国际病理科学与临床杂志,2017,37(5):925-931,7.
