南方农业学报2017，Vol.48Issue(6)：1062-1067,6.DOI:10.3969/j.issn.2095-1191.2017.06.20
超声辅助酶重量法提取树仔菜总膳食纤维
Extraction of total dietary fiber from Sauropus androgynus L. Merr by ultrasonic-assisted enzymatic-gravimetric method
摘要
Abstract
[Objective]The extraction process of total dietary fiber from Sauropus androgynus L. Merr by ultrasonic-as-sisted enzymetic-gravimetric method was optimized, in order to provide a theoretical reference for researching dietary fiber of wild vegetable. [Method]Using S. androgynus L. Merr as materials, the effects of protease types, enzyme amount, so-lution pH, ultrasonic time and ultrasonic temperature on extraction rate of total dietary fiber from S. androgynus L. Merr were investigated by single-factor test. Meanwhile, based on the results of single-factor test, the extraction technology was optimized using orthogonal experiment. [Result]Effects of protease types on extraction efficiency were ranked as follows:papain>neutral protease>alkaline protease>trypsin. The optimal technological parameters for ultrasound-assisted enzyma-tic-gravimetric extraction of S. androgynus L. Merr total dietary fiber were as follows: papain amount 0.60% , solution pH 5.0, ultrasonic time 50 min and ultrasonic temperature 65 ℃. Extraction efficiency was effected by papain>ultrasonic time>ultrasonic temperature>solution pH. Under the above optimum conditions, the extraction rate of total dietary fiber from S. androgynus L. Merr reached up to 45.0%, which was close to the value(44.8%) of GB 5009.88-2014 Measure-ment of Dietary Fiber in Food. The coefficient of variation was less than 5.00%. [Conclusion]The optimized extraction process by ultrasonic-assisted enzymetic-gravimetric method shortens enzymolysis time, and is easy to operate, hence it can be applied in scale extraction of total dietary fiber from samples.关键词
树仔菜/膳食纤维/酶重量法/超声辅助Key words
Sauropus androgynus L. Merr/dietary fiber/enzymetic-gravimetric method/ultrasonic-assisted分类
轻工纺织引用本文复制引用
邓爱妮,范琼,周聪,赵敏..超声辅助酶重量法提取树仔菜总膳食纤维[J].南方农业学报,2017,48(6):1062-1067,6.基金项目
海南省自然科学基金项目(214036,814305) （214036,814305）
海南省重点科技计划项目(ZDXM2014135) （ZDXM2014135）
