广西医学2017，Vol.39Issue(7)：1029-1031,1034,4.DOI:10.11675/j.issn.0253-4304.2017.07.29
肢体缺血后处理通过Nrf2/ARE通路对大鼠脑缺血再灌注损伤的影响
Effect of limb ischemic post-conditioning on rats with cerebral ischemia-reperfusion injury through Nrf2/ARE pathway
摘要
Abstract
Objective To investigate the effect of limb ischemic post-conditioning on rats with cerebral ischemia-reperfusion injury(CIRI) through nuclear factor erythroid 2-related factor 2(Nrf2)/antioxidant response element(ARE) pathway.Methods A total of 45 rats were randomly divided into sham-operation group(Group S),ischemia-reperfusion group(Group I/R) and limb ischemic post-conditioning group(LpostC group),with 15 cases in each group.The limb ischemic post-conditioning models were established in LpostC group on the basis of the cerebral ischemia-reperfusion models established in Group I/R and LpostC group respectively.After 24 hours of modeling,the neurological function defect score,cerebral infarction volume,pathomorphological changes in brain tissues,protein expressions of Nrf2 and superoxide dismutase(SOD)1 were compared among the three groups respectively.Results There were no neurological function defect or cerebral infarction in Group S,the neurological function defect score and cerebral infarct volume of the LpostC group were less than those of Group I/R(all P<0.05).There were no pathological changes in brain tissues in Group S,acute ischemic changes were found in Group I/R,and the pathological changes in LpostC group were milder than those in Group I/R;the protein expressions of Nrf2 and SOD1 in Group I/R and LpostC group were higher than those in Group S,and the expressions in LpostC group were higher than those in Group I/R(all P<0.05).Conclusion Limb ischemia post-conditioning can up-regulate the protein expressions of Nrf2 and SOD1 in rats and relieve CIRI through activating the Nrf2/ARE signaling pathway.关键词
肢体缺血后处理/脑缺血再灌注损伤/Nrf2/ARE通路/超氧化物歧化酶11Key words
Limb ischemic post-conditioning/Cerebral ischemia-reperfusion injury/Nuclear factor erythroid 2-related factor 2/antioxidant response element pathway/Superoxide dismutase 1分类
医药卫生引用本文复制引用
安祥,阳昀,刘开祥,李浩,冷光朋,李偲,廖小明..肢体缺血后处理通过Nrf2/ARE通路对大鼠脑缺血再灌注损伤的影响[J].广西医学,2017,39(7):1029-1031,1034,4.基金项目
广西自然科学基金(2013GXNSFAA019190) （2013GXNSFAA019190）
广西医药卫生科研课题(Z2014338) （Z2014338）
