工业微生物2017，Vol.47Issue(3)：9-13,5.DOI:10.3969/j.issn.1001-6678.2017.03.002
β-胡萝卜合成途径的不同启动子组合的比较研究
Comparison of promoters' efficiency in β-carotene biosynthetic pathway
童阳阳 1高书良 2蒋宇 3杨晟 3戈梅 4陈代杰5
作者信息
- 1. 上海师范大学生命与环境科学学院,上海200234
- 2. 华东理工大学生物工程学院,上海200237
- 3. 中国科学院上海生命科学研究院,上海200031
- 4. 上海来益生物药物研究开发中心,上海201203
- 5. 上海医药工业研究院,上海200040
- 折叠
摘要
Abstract
Yarrowia lipolytica MYA2613 was used as a host to efficiently produce β-carotene with expression of exogenetic genescarB and carRP from Mucorcircinelloides.Pathway could be optimized through dynamic regulation of genes expression.Here,two sets of promoters which control three genes of β-carotene biosynthetic pathway,TGE,TEF1p-GGS1-xpr2t-GPD1 p-carB-mig1 t-EXP1 p-carRP-lip2t and YGF,YAT1 p-GGS1-xpr2t-GPD1 p-carB-mig1 t-FBAin1p-carRP-lip2t.Strains carrying each of these two pathways were investigated through fermentation.Results indicated that TGE was a better combination of promoters to express β-carotene pathway.The production of β-carotene by TGE strains was 4.1-fold of that by YGF strains.关键词
β-胡萝卜素/DNA assemble/耶氏解脂酵母/启动子组合Key words
β-carotene/DNA assemble/Yarrowia lipolytica/promoter combination引用本文复制引用
童阳阳,高书良,蒋宇,杨晟,戈梅,陈代杰..β-胡萝卜合成途径的不同启动子组合的比较研究[J].工业微生物,2017,47(3):9-13,5.
