工业微生物2017，Vol.47Issue(3)：14-21,8.DOI:10.3969/j.issn.1001-6678.2017.03.003
一株耐受低pH、高浓度硒菌株的筛选鉴定及两阶段pH调控高效除硒的研究
Isolation and identification of a low-pH and selenium tolerant strain and research on efficient removal of selenium using two-stage pH control strategy
摘要
Abstract
The strain Y1 with low pH and high concentration of selenium tolerant was screened by acidic selenium-containing plate and shake flask.This strain was identified as Pichia kudriavzevii by 26S rDNA sequence and morphology characteristics.Multi-stress test results showed that the strain could be described as a multi-stress-tolerant yeast.The effects of temperature,inoculation amount,shaking speed and pH on the selenium removal performance of the strain was studied by shake flask experiment.The results showed that the maximum removal rate of selenium was under the following conditions:temperature 25 ℃,inoculation amount 12％ (v/v),shaking speed 250 r/min and pH 3.0.Based on specific biomass and specific Se bioaccumulation under different pH conditions:there was the maximum biomass at pH 3.0 and the maximum Se bioaccumulation at pH 5.0.A two-stage pH control strategy was therefore developed,i.e.pH was controlled at 3.0 in the first stage of fermentation (0 h ～14 h) and then switched to pH 5.0 in the second stage (14 h ～28 h).The final removal rate could reach to 78.6％.It was 15.4％ higher than that of pH 3.0 and 21.7％ higher than that of pH 5.0,respectively.关键词
耐酸/耐硒/筛选与鉴定/除硒/两阶段pHKey words
acid tolerance/selenium tolerance/screening and identification/removal of selenium/two-stage pH引用本文复制引用
陈纪龙,朱莉,高敏杰,詹晓北,黄艳艳..一株耐受低pH、高浓度硒菌株的筛选鉴定及两阶段pH调控高效除硒的研究[J].工业微生物,2017,47(3):14-21,8.基金项目
国家自然科学基金(31301408),中央高效基本科研业务费专项资金资助(JUSRP51632A、JUSRP51504) （31301408）
江苏省政策引导类计划(产学研合作)-前瞻性联合研究项目(BY2016022-15). （产学研合作）
