工业微生物2017，Vol.47Issue(3)：22-29,8.DOI:10.3969/j.issn.1001-6678.2017.03.004
以甘油为唯一碳源的氧化葡糖杆菌适应性驯化及催化合成米格列醇中间体6NSL
Use of glycerol as sole carbon source for G.oxydans adaptation domestication and bioconversion of miglitol intermediate 6NSL
摘要
Abstract
Sorbitol dehydrogenase (SLDH),originated from Gluconobacter oxydans,is capable to catalyze N-hydroxyethyl glucamine into 6-deoxy-6-amino (N-hydroxyethyl)-α-L-furansorbose (6NSL),which is a key intermediate for synthesis of the hypoglycemic drug miglitol.A high-activity strain G.oxydans A-3-D was obtained by glycerol gradient adaptation with 40 g/L,60 g/L,80 g/L,100 g/L as sole carbon source.SEM observation demonstrated that the plasma membrane folds in cell surface area was significantly increased in G.oxydans A-3-D if compared with that of the original strain G.oxydans ZJB-605.After 24 h cultivation in 80 g/L glycerol medium,4.58 g DCW/L cells were obtained in G.oxydans A-3-D and the catalytic activity of SLDH was significantly increased by 1.3-folds for total enzymatic activity and 1.5-folds for specific enzymatic activity,respectively,compared with that of G.oxydans ZJB-605.In addition,key parameters involved in bioconversion processes were investigated.Based on the data obtained under the optimized catalytic conditions in shake-flask (including 80 g/L substrate,2.0 gDCW/L cell and 20 mmol/L Mg2 +),the conversion rate reached to 90.8％ and 72.6 g/L 6NSL was accumulated within 48 h at 15 ℃2,which was significantly enhanced if compared with that of G.oxydans ZJB-605 strain.关键词
氧化葡糖杆菌/米格列醇/山梨醇脱氢酶/甘油/适应性驯化Key words
Gluconobater oxydans/miglitol/SLDH/glycerol/adaptation引用本文复制引用
柯霞,汪宁宁,鲁阳辉,胡忠策..以甘油为唯一碳源的氧化葡糖杆菌适应性驯化及催化合成米格列醇中间体6NSL[J].工业微生物,2017,47(3):22-29,8.基金项目
浙江省教育厅科研项目Y201329109. （）
