工业微生物2017，Vol.47Issue(3)：30-35,6.DOI:10.3969/j.issn.1001-6678.2017.03.005
产生物活性物质海洋放线菌的筛选和鉴定
Screening and identification of active substances from Marine actinomycetes
摘要
Abstract
In this study,38 marine actinomycetes were isolated from samples of sea water,sea mud and sea cucumber farming circles in Dalian sea area.Five indicators such as bacteria,Staphylococcus aureus,Micrococcus lysodeikticus,Bacillus subtilis,Vibrio parahaemolyticus and Pseudomonas aeruginosa were chosen for screening.And two strains named as HS-B31 and HS-B34 exhibited strong antibacterial activity.Both strains were identified by 16S rDNA sequence,Blast and phylogenetic analysis.The results indicated that the two marine actinomycetes belonged to different Streptomyces species.The fermentation supernatants of the two strains were extracted by ethyl acetate.The crude extracts were concentrated to obtain the crude substances,named as B31 and B34.It was also found that both substances showed remarkable antibacterial activity.The active substances were isolated and analyzed by thin-layer chromatography.Five components (B31-1,B31-2,B31-3,B31-4 and B31-5) were isolated from B31,and three components (B34-1、B34-2、B34-3) were isolated from B34.Then all components of antibacterial activity were analyzed.The results revealed that the compounds B31-3 and B34-3 had stronger antibacterial activity against the gram-positive bacterium of V.parahaemolyticus and the gram-negative bacterium of S.aureus.These results would provide new microorganism resources for the development and application of antibiotics.关键词
海洋放线菌/16S rDNA/鉴定/抗菌活性物质Key words
Marine actinomycetes/16S rDNA/identification/antibacterial activity substance引用本文复制引用
王艺颖,丛丽娜,王伟,孙珊,于咏梅,孙野,李秋月,李成..产生物活性物质海洋放线菌的筛选和鉴定[J].工业微生物,2017,47(3):30-35,6.基金项目
辽宁省自然科学基金项目(201602047),国家海洋公益性行业科研专项项目(201405003),辽宁省教育厅重点实验室(LZ2014029). （201602047）
