工业微生物2017，Vol.47Issue(3)：36-42,7.DOI:10.3969/j.issn.1001-6678.2017.03.006
响应面法对微拟球藻产微藻蛋白的发酵条件优化
Optimization of fermentation conditions for protein production of Nannochloropsis gaditana by response surface methodology
摘要
Abstract
In order to improve microalgae protein productivity during stable phase,the response surface methodology method was used to optimize the fermentation conditions of batch cultivation of Nannochloropsis gaditana.Based on the data from single-factor trial,main factors including the temperature,pH,stirring speed and aeration rate were selected as influencing factors,and then the experiments with four factors and three levels were designed according to a Box-Benhnken central composite rule.The results showed that the optimal fermentation conditions were as follows:temperature 30 ℃,pH 6.9,stirring speed 340 r/min and aeration rate 0.65 vvm.Finally,the confirmatory experiment was performed under these optimized conditions.The final protein concentration was as high as 6.18 g/L,increased by 9.18％ than before.This result was consistent with the model prediction.关键词
微拟球藻/微藻蛋白/响应面法Key words
Nannochloropsis gaditana/microalgae protein/response surface methodology引用本文复制引用
王怡梅,周鑫鑫,邓刚..响应面法对微拟球藻产微藻蛋白的发酵条件优化[J].工业微生物,2017,47(3):36-42,7.基金项目
国家自然科学基金(No.21006098). （No.21006098）
