海南医学院学报2017，Vol.23Issue(11)：1452-1455,4.DOI:10.13210/j.cnki.jhmu.20170518.003
骨关节炎大鼠模型软骨组织中PI3K/AKT信号通路功能与细胞凋亡的相关性研究
Relationship between PI3K/AKT signaling pathway and apoptosis in cartilage tissue of rats with osteoarthritis
摘要
Abstract
Objective: To study the relationship between PI3K/AKT signaling pathway and apoptosis in cartilage tissue of rats with osteoarthritis (OA).Methods: The clean male Wistar rats were selected as experimental animals and randomly divided into OA model group and control group.The OA model was established by intra-articular injection of papain solution and L-cysteine.Fourth weeks and eighth weeks after model establishment, the expression of PI3K/AKT signaling molecules, inflammatory mediators, apoptosis marker molecules and autophagy marker molecules in articular cartilage were determined.Results: p-PI3K and p-AKT expression in articular cartilage of OA group were significantly lower than those of control group;IL-1β, IL-6, IL-17, IL-18, eIF4E, Bax, Caspase-3, mTOR, Beclin1, Atg5 and Atg7 expression in articular cartilage of OA group were significantly higher than those of control group and negatively correlated with p-PI3K and p-AKT expression while Bcl-2 expression in articular cartilage of OA group was significantly lower than that of control group and positively correlated with p-PI3K and p-AKT expression.Conclusions: The inhibition of PI3K/AKT signaling pathway in cartilage tissue of OA rat model can promote chondrocyte apoptosis and autophagy.关键词
骨关节炎/PI3K/AKT/白细胞介素/凋亡/自噬Key words
Osteoarthritis/PI3K/AKT/Interleukin/Apoptosis/Autophagy分类
医药卫生引用本文复制引用
李晓燕,张艳,董春萍,吴贵福..骨关节炎大鼠模型软骨组织中PI3K/AKT信号通路功能与细胞凋亡的相关性研究[J].海南医学院学报,2017,23(11):1452-1455,4.基金项目
陕西省自然科学基础研究计划项目(2016JQ8055)It was supported by Natural Science Basic Research Project of Shaanxi Province (No.2016JQ8055). （2016JQ8055）
