海南医学院学报2017，Vol.23Issue(11)：1456-1458,1462,4.DOI:10.13210/j.cnki.jhmu.20170525.011
血管紧张素1-7对氧化应激所致内皮细胞损伤的影响
Effect of angiotensin 1-7 on endothelial cell injury caused by oxidative stress
摘要
Abstract
Objective: To study the effects of angiotensin 1-7 (Ang1-7) on endothelial cell injury caused by oxidative stress.Methods: Human umbilical vein endothelial cells (HUVECs) were cultured and divided into blank control group, hydrogen peroxide and different Ang1-7 dose groups (1 μmol/L, 2 μmol/L and 4 μmol/L Ang1-7 groups).The cell proliferation activity, the contents of antioxidant enzymes in cell culture medium, and the contents of endoplasmic reticulum stress molecules in cells were determined.Results: After 6, 12, 18 and 24 h of treatment, CCK-8 proliferation activity of hydrogen peroxide group were significantly lower than those of blank control group, CCK-8 proliferation activity of 1 μmol/L, 2 μmol/L and 4 μmol/L Ang1-7 groups were significantly higher than those of hydrogen peroxide, and the larger the Ang1-7 dose, the higher the CCK-8 proliferation activity;after 24 hours of treatment, SOD, GSH-Px, HO-1 and CAT contents in cell culture medium of hydrogen peroxide group were significantly lower than those of control group, as well as GRP78, XBP1 and CHOP contents in cells were significantly higher than those of control group;SOD, GSH-Px, HO-1 and CAT contents in cell culture medium of 1 μmol/L, 2 μmol/L and 4 μmol/L Ang1-7 groups were significantly higher than those of hydrogen peroxide group, as well as GRP78, XBP1 and CHOP contents in cells were significantly lower than those of hydrogen peroxide group, and the larger the Ang1-7 dose, the more significant the changes of above molecules in cell culture medium and cells.Conclusions: Angiotensin 1-7 has protective effect on the endothelial cell injury caused by oxidative stress.关键词
血管紧张素1-7/氧化应激/内皮细胞/内质网应激Key words
Angiotensin 1-7/Oxidative stress/Endothelial cell/Endoplasmic reticulum stress分类
医药卫生引用本文复制引用
吴中俭,屈海龙..血管紧张素1-7对氧化应激所致内皮细胞损伤的影响[J].海南医学院学报,2017,23(11):1456-1458,1462,4.基金项目
北京市医管局登峰人才项目(DFL20150801) It is supported by Dengfeng Talents Project of Beijing Municipal Administration of Hospitals(DFL20150801). （DFL20150801）
