华北农学报2017，Vol.32Issue(3)：1-6,6.DOI:10.7668/hbnxb.2017.03.001
水稻阶段性温敏白化转绿突变体stgra254的特征和基因定位
Characterization and Gene Mapping of Stage Thermo-sensitive Green-revertible Albino Mutant stgra254 in Rice
摘要
Abstract
In order to explore and identify more albino mutants for studying gene functions,a stage thermos-sensitive green-revertible albino mutant in rice,designated as stgra254,was isolated by treating the seeds of japonica variety Xiushui 09 using EMS.The phenotype of gra254 is thermos-sensitive.Under a constant temperature of 28 ℃,the white spots appeared on the fully expanded sixth leaves,and gradually enlarged and merged into one at maximum tillering stage.Then the leaves withered to death.The sixth leaf blades showed milder expression of the mutant phenotype under 32 ℃ condition than that under 28 ℃ condition,and gradually turned green from the third day onward.Under 24 ℃ condition,however,the gra254 plants produced normal green leaves.Histochemical staining analysis indicated that the albino trait of stgra254 was caused by the programmed cell death,which probably caused by oxidative burst accompanying by the accumulation of H2O2.Analysis by fluorescence analyzer presented that the maximal photochemical efficiency of PS Ⅱ decreased significantly.Genetic analysis indicated that the thermos-sensitive green-revertible albino trait was controlled by a single recessive nucleic gene.Based on the F2 population derived from a cross between stgra254 and Zhenshan 97,the stgra254 was located between the SSR markers of RM17206 and RM17277 on chromosome 4 by bulked segregant analysis and linkage analysis.The genetic distance was 0.48,5.22 cM, respectively.关键词
水稻/白化转绿基因/温敏/基因定位Key words
Rice/Green-revertible albino/Thermos-sensitive/Gene mapping分类
生物科学引用本文复制引用
邹金财,张维林,夏明辉,邱洋松,王长春,杨玲,张小明..水稻阶段性温敏白化转绿突变体stgra254的特征和基因定位[J].华北农学报,2017,32(3):1-6,6.基金项目
浙江省水稻种业科技创新团队项目(2010R50024) （2010R50024）
