河北医学2017，Vol.23Issue(7)：1091-1094,4.DOI:10.3969/j.issn.1006-6233.2017.07.011
联合应用重组人血管内皮抑素与紫杉醇对乳腺癌细胞株SK-BR-3诱导凋亡作用研究
Apoptosis Induced by Recombinant Human Endostatin and Paclitaxel in Breast Cancer Cell Line SK-BR-3
摘要
Abstract
Objective:To observe the growth and apoptosis rate of recombinant human endostatin combined with paclitaxel in breast cancer cell line SK-BR-3.Methods: The growth of cells was observed under light microscope.The apoptosis rate of breast cancer cell SK-BR-3 was detected by flow cytometry.The expression of apoptosis related protein Bcl-2/Bax was observed by Realtime PCR method.The apoptosis inducing effects of different administration protocols on breast cancer cell line SK-BR-3 were compared.Results: Apoptosis of Endostar 1 g/ml after 48h was 11.6% (P<0.05), Bcl-2 expression, and the expression of Bax was not changed.The rate of apoptosis was 12.4% (P<0.05) after paclitaxel was 1 g/ml, and the expression of Bcl-2 was down regulated, while the expression of Bax was increased by 24h.Paclitaxel and sequential administration of Endostar respectively, the apoptosis rates were 16.50% and 14.18% (P<0.05), and can down regulate the expression of Bcl-2, increase the expression of Bax.The apoptosis rate of SK-BR-3 cells was 22.74%, which was significantly higher than that of other administration group (P<0.05), and the expression of Bcl-2 was down regulated and the expression of Bax was elevated at the same time when the two groups were combined and administered at the same time.Conclusion: Both endostar and paclitaxel can inhibit the growth of breast cancer cell line SK-BR-3 and induce apoptosis.The two drugs simultaneously produced the best effect.关键词
恩度/紫杉醇/乳腺癌/SK-BR-3细胞/凋亡Key words
Endostar/Paclitaxel/Breast cancer/SK-BR-3 cells/Apoptosis引用本文复制引用
王晓利,王晓成,徐晓明,杜建文,姜浩..联合应用重组人血管内皮抑素与紫杉醇对乳腺癌细胞株SK-BR-3诱导凋亡作用研究[J].河北医学,2017,23(7):1091-1094,4.基金项目
河北省医学科学研究重点课题,(编号:20160306) （编号:20160306）
