郑州大学学报（医学版）2017，Vol.52Issue(4)：377-380,4.DOI:10.13705/j.issn.1671-6825.2017.04.001
α-细辛醚对Eca-109细胞凋亡及XIAP、caspase-3表达的影响
Effect of α-asarone on apoptosis of Eca-109 cells and expressions of XIAP and caspase-3
摘要
Abstract
Aim:To observe the apoptosis-inducing effect of α-asarone on esophageal carcinoma Eca-109 cells and changes of the expressions of XIAP and caspase-3,and explore the underlying mechanism.Methods: Eca-109 cells were allocated into 5 groups,and treated with 5-FU(positive control group),25,50,100 mg/L α-asarone(low-,medium-,and high-dose α-asarone groups) and culture medium(blank control group),respectively.After 48 h,morphologic changes were observed under inverted fluorescence microscope after AO/EB staining.MTT and Annexin-V/PI method were used to detect cell proliferation and apoptosis.Western blot and real-time quantitative PCR were adopted to detect XIAP and caspase-3 mRNA and protein expressions in blank control group,medium-dose α-asarone group and high-dose α-asarone group.Results: Compared with blank control group,cell proliferation rate in different doses of α-asarone groups and positive control group decreased significantly,while cell apoptosis rate increased significantly(P<0.05).In contrast with blank control group,the expressions of XIAP mRNA and protein decreased significantly,while those of caspase-3 increased significantly in medium-and high-dose α-asarone groups(P<0.05).Conclusion: α-asarone could inhibit Eca-109 cells proliferation and induce cell apoptosis by down-regulating XIAP expression and up-regulating caspase-3 expression.关键词
α-细辛醚/X连锁凋亡抑制蛋白/caspase-3/凋亡/Eca-109细胞Key words
α-asarone/X-linked inhibitor of apoptosis protein/caspase-3/apoptosis/Eca-109 cell分类
医药卫生引用本文复制引用
张妍,何航,韩倩倩,王白燕,朱艳琴..α-细辛醚对Eca-109细胞凋亡及XIAP、caspase-3表达的影响[J].郑州大学学报（医学版）,2017,52(4):377-380,4.基金项目
河南省基础与前沿技术研究项目 072300450030 （）
河南省科学技术研究重点项目 13A310612 （）
郑州市科技攻关项目 121PCXTD520 （）
河南省教育厅高等学校重点科研项目 16A310021 （）
