郑州大学学报（医学版）2017，Vol.52Issue(4)：393-398,6.DOI:10.13705/j.issn.1671-6825.2017.04.005
染料木黄酮对去势抵抗前列腺癌22RV1细胞增殖的影响
Effects of genistein on proliferation of castrate-resistant prostate cancer 22RV1 cells
摘要
Abstract
Aim:To explore the effect of soy isoflavones,genistein on the proliferation of castration-resistant prostate cancer 22RV1 cells and its potential mechanism.Methods: 22RV1 cells were treated with genistein at 0.0,12.5,25.0,50.0,100.0,and/or 200.0 μmol/L for 24,48,72 hours followed by determination of their proliferation by CCK-8 assay,and their cell cycle by flow cytometry.The expression of Ki-67 was detected by immunocytochemistry.The expressions of PSA,CyclinD1,PCNA,P53 and AR protein were detected by Western blot.Results: Genistein had inhibitory effect on 22RV1 cells.Flow cytometry results showed that the cell cycle in the genistein treated groups was arrested at G2/M phase(P<0.05).The expression of Ki-67 in the genistein-treated groups was significantly lower than that in the control group(P<0.05).The results of Western blot showed that genistein could reduce CyclinD1,PCNA,PSA,and AR expressions,and increase P53 expression(P<0.05).Conclusion: Genistein could inhibit the proliferation of 22RV1 cells via arresting the cell cycle and reducing the expression of CyclinD1.关键词
染料木黄酮/去势抵抗前列腺癌/增殖/22RV1细胞Key words
genistein/castrate-resistant prostate cancer/proliferation/22RV1 cell分类
医药卫生引用本文复制引用
李飞,朱彦锋,陈静瑶,周杰,贺易琼,余小平..染料木黄酮对去势抵抗前列腺癌22RV1细胞增殖的影响[J].郑州大学学报（医学版）,2017,52(4):393-398,6.基金项目
国家自然科学基金资助项目 81402678,81573154 （）
四川省青年科技创新研究团队项目 2014TD0021 （）
成都医学院大学生创新实验项目 201513705027 （）
