郑州大学学报（医学版）2017，Vol.52Issue(4)：412-415,4.DOI:10.13705/j.issn.1671-6825.2017.04.009
microRNA-509-3p对肝癌HepG2细胞增殖和侵袭能力的影响
Effects of microRNA-509-3p on proliferation and invasiveness of liver carcinoma HepG2 cells
摘要
Abstract
Aim:To explore the role of microRNA(miR)-509-3p in proliferation and invasion of liver carcinoma cells.Methods: A total of 60 cases of liver carcinoma tissue and 60 cases of paracancerous tissue were selected,and the miR-509-3p expression was detected by real-time PCR.HepG2 cells were cultured and transfected with miR-509-3p mimic,miR-509-3p inhibitor or XIAP siRNA,and cells without any treatment were the control group.The cell proliferation activity was detected by CCK-8,and cell invasion was detected by Transwell cell migration assay.Results: The expression of miR-509-3p in liver carcinoma tissue was significantly lower than that in paracancerous tissue(P<0.001).Compared with the control group,the cell proliferation rate and the number of migration cells in miR-509-3p mimic group were significant lower(P<0.05),while those in miR-509-3p inhibitor group were higher(P<0.05),and miR-509-3p overexpression contributed to the inhibition of XIAP expression(P<0.05).Conclusion: miR-509-3p plays an important role in the development and progression of liver carcinoma,and its downregulation of miR-509-3p may be closely associated with the high expression of XIAP.关键词
肝癌/HepG2细胞/微小RNA-509-3p/XIAP/细胞侵袭/增殖Key words
liver carcinoma/HepG2 cell/microRNA-509-3p/XIAP/cell invasion/proliferation分类
医药卫生引用本文复制引用
程豪为,杨荟玉,杨君霞,孙爱民,陈宏涛,杨小昂,巴秋菊..microRNA-509-3p对肝癌HepG2细胞增殖和侵袭能力的影响[J].郑州大学学报（医学版）,2017,52(4):412-415,4.基金项目
河南省基础与前沿项目 142300410326 （）
