郑州大学学报（医学版）2017，Vol.52Issue(4)：424-427,4.DOI:10.13705/j.issn.1671-6825.2017.04.012
盐酸米诺环素联合栀子苷对NO诱导PC12细胞凋亡的影响
Effects of minocycline combined with geniposide on apoptosis of PC12 cells induced by nitric oxide
摘要
Abstract
Aim:To investigate the effects of minocycline,geniposide alone or combined on the apoptosis of PC12 cells induced by nitric oxide and the possible mechanism.Methods: PC12 cells were divided into control group,minocycline group,geniposide group and the combined group.The four groups were all given 0.5 mmol/L SIN-1 to induce apoptosis,minocycline group was given 0.1 mmol/L minocycline 12 hours before the SIN-1 intervention,geniposide group was given 0.25 mmol/L geniposide,the combined group was given the 2 drugs,and control group was not given any drug.The cell survival was determined by CCK-8 method,the apoptosis was observed by Hoechest 33342 fluorescent staining,the apoptosis rate was measured by flow cytometry,the expressions of the apoptotic related proteins Caspase-3 and Bcl-2 were detected by Western blot.Results: Compared with control group,the cell viability improved,the apoptosis rate decreased,the expression of Bcl-2 upregulated,and that of Caspase-3 downregulated in minocycline group and geniposide group(P<0.05);minocycline and geniposide had synergistic effects on the above indicators(P<0.05).Conclusion: Minocycline and geniposide both can inhibit the apoptosis of PC12 cells,but the effect of the combined application is superior to the single application.The mechanism may be related to the inhibition of Caspase-3 dependent apoptosis signaling pathways.关键词
盐酸米诺环素/栀子苷/PC12细胞/凋亡/Caspase-3/Bcl-2Key words
minocycline/geniposide/PC12 cell/apoptosis/Caspase-3/Bcl-2分类
医药卫生引用本文复制引用
蔡智慧,王晋,吴琼,常全忠..盐酸米诺环素联合栀子苷对NO诱导PC12细胞凋亡的影响[J].郑州大学学报（医学版）,2017,52(4):424-427,4.基金项目
河南省科技厅基础与前沿技术研究基金资助项目 152300410014 （）
河南省教育厅高等学校重点科研项目 16A180031 （）
漯河医学高等专科学校博士基金资助项目 2014DF001 （）
漯河医学高等专科学校校级研究项目 2016-S-LMC-9 （）
