郑州大学学报（医学版）2017，Vol.52Issue(4)：460-462,3.DOI:10.13705/j.issn.1671-6825.2017.04.021
假肥大肌营养不良症患儿腓肠肌组织中抗肌萎缩蛋白相关蛋白mRNA表达的检测
Detection of mRNA expression of Utrophin in gastrocnemius muscle tissue of patients with pseudohypertrophy muscular dystrophy
摘要
Abstract
Aim:To investigate the expression level of Utrophin in the gastrocnemius muscle tissue of patients with Duchenne and Becker muscular dystrophy(DMD and BMD).Methods: A total of 24 patients with DMD(DMD group) and 7 patients with BMD(BMD group) were chosen,and 21 healthy male children were chosen as control.The DMD group was further allocated into DMD1 group(n=6),DMD2 group(n=12),and DMD3 group(n=6).The expression levels of Utrophin and Dystrophin mRNA in the above groups were detected by real-time quantitative PCR.The relationship between Utrophin mRNA and Hammersmith functional motor score(HFMS) of the patients with muscular dystrophy was analyzed.Results: Compared with the control group,Utrophin mRNA significantly over-expressed in the muscular dystrophy group(P=0.002).The expression level of Utrophin mRNA in the gastrocnemius muscle in the DMD group was significantly higher compared with BMD group(P=0.003);compared with BMD1 group,the expression level of Utrophin mRNA in BMD2 group and BMD3 group was significantly higher,especially in DMD3 group(P<0.05).There was a positive correlation between Utrophin mRNA expression and HFMS in patients with DMD or BMD(rS=0.671,P=0.003).Conclusion: Utrophin expression level in gastrocnemius muscle tissue from the patients with muscular dystrophy rises.Utrophin could replace part of Dystrophin function in the mature skeletal muscle,which may play a role in delaying the progression of muscle weakness.关键词
肌营养不良症/腓肠肌/抗肌萎缩蛋白相关蛋白/肌无力Key words
muscular dystrophy/gastrocnemius muscle/Utrophin/myasthenia分类
医药卫生引用本文复制引用
崔明,刘恒方,张敏,宋长栋,王建平..假肥大肌营养不良症患儿腓肠肌组织中抗肌萎缩蛋白相关蛋白mRNA表达的检测[J].郑州大学学报（医学版）,2017,52(4):460-462,3.基金项目
河南省科技厅重点资助项目 132102310092 （）
