河南农业大学学报2017，Vol.51Issue(3)：324-329,6.
菊花去饱和酶CmSAD基因的克隆与表达分析
Cloning and express analysis of fatty acid desaturase gene CmSAD in chrysanthemum
摘要
Abstract
Chrysanthemum (Chrysanthemum morifolium Ramat.) variety of cold resistance ‘ Xingguangcanlan’ was selected as experimental materials.△9 Stearoyl-ACP desaturase (SAD) gene were separated and cloned from the leaf by RT-PCR and RACE,named CmSAD,accession number is KC529335 in GenBank.The full length cDNA of CmSAD is 1203 bp,encoding a protein of 401 amino acids.Its molecular mass is 45.57 kD,pI is 6.48,and the homology of coding sequence of amino acids is the highest with Saussurea involucrata(80.29％).This protein has no transmembrane structure,and mainly exists in the chloroplast stroma,and a chloroplast transit peptide at N-terminal,which contains 60 amino acids.Quantitative PCR research by real-time fluorescent was conducted on quantity alteration in leaves and root under low temperature stress CmSAD gene expression.The expression quantity of CmSAD gene decreased in root system with temperature decreasing.When the temperature was 16 ℃,the expression quantity of CmSAD gene was the highest.With the decrease of temperature,the expression quantity of CmSAD gene in leaves increased first and achieved the highest at 5 ℃,then decreased.Different temperatures made diffident expression quantity of CmSAD gene.With the decrease of temperature,the expression quantity of CmSAD gene decreased.Therefore,the expression quantity of CmSAD gene was related to temperature and it also had a close relationship with cold resistance.关键词
菊花/低温/CmSAD基因/克隆/表达分析Key words
chrysanthemum/low temperature/CmSAD/cloning/expression analysis分类
农业科技引用本文复制引用
张华奥,逯久幸,李静,李永华..菊花去饱和酶CmSAD基因的克隆与表达分析[J].河南农业大学学报,2017,51(3):324-329,6.基金项目
河南省科技攻关项目(142102110136) （142102110136）
