环境工程学报2017，Vol.11Issue(7)：4419-4428,10.DOI:10.12030/j.cjee.201604200
1株异养硝化好氧反硝化不动杆菌的分离及脱氮性能
Isolation of Acinetobacter sp.YN3 and its Heterotrophic nitrification-aerobic denitrification characters
摘要
Abstract
An efficient heterotrophic nitrification bacteria species was isolated from activated sludge through enrichment and cultured with a heterotrophic nitrification medium.The bacteria was identified as Acinetobacter sp.by morphological,physiological,and biochemical characteristics,as well as 16S rDNA sequence analysis.This strain was named Acinetobacter sp.YN3.Single-factor and orthogonal experiments showed that efficient heterotrophic nitrification of strain YN3 occurred with sodium citrate as the carbon source,a C/N ratio of 15,pH of 7.0,temperature of 30 ℃,and a rotating speed of 200 r · min-1.When initial ammonia was 300 mg · L-1,the ammonia removal efficiency reached 99.3％,of which 37.8％ was converted to gas and escaped to air,and the rest was fixed to cell biomass.Using nitrite and nitrate as a nitrogen source,the nitrogen degradation ratios were 100％ and 80.6％,respectively.It was indicated that strain YN3 could remove nitrogen through heterotrophic nitrification-aerobic denitrification quickly and effectively.This strain therefore has good potential for use in waste water treatment.关键词
异养硝化/好氧反硝化/生物脱氮/不动杆菌/正交实验Key words
heterotrophic nitrification/aerobic denitrification/biological removal of nitrogen/orthogonal experiments/Acinetobacter sp.分类
资源环境引用本文复制引用
颜薇芝,张汉强,余从田,雷艳芳,郝健,史吉平..1株异养硝化好氧反硝化不动杆菌的分离及脱氮性能[J].环境工程学报,2017,11(7):4419-4428,10.基金项目
上海市农委项目资助(沪农科攻字(2015)第1-4号) （沪农科攻字(2015）
