环境工程学报2017，Vol.11Issue(7)：4429-4436,8.DOI:10.12030/j.cjee.201605142
基于间接竞争抑制ELISA的2,2',4,4'-四溴联苯醚检测方法
Resarch of 2,2',4,4'-Tetrabromodiphenyl Ether detection method based on indirect competitive inhibition enzyme-linked immunosorbent assay
摘要
Abstract
The existing methods for the detection of polybrominated diphenyl ethers (PBDEs) in complex sam ples face challenges like high costs and long sample testing times.This study assessed the indirect competitive inhibition enzyme-linked immunosorbent assay (ELISA) of 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) as a representative PBDE,comparing performance with those of gas chromatography-mass spectrometry (GC-MS) de tection methods and ELISA kits.For the indirect competitive inhibition ELISA method,the value of IC50 was 0.11 mg · L-1,the limit of quantification was 0.04 mg · L-1,and the detection range was 0.01-0.50 mg · L-1.Meeting the requirements of sensitivity and reproducibility of detecting trace targets in a complicated matrix,ELISA is a more efficient,economic,and practical method suitable for early screening of large quantities of envi ronmental samples.关键词
多溴联苯醚/酶联免疫吸附分析/间接竞争抑制/气相色谱-质谱Key words
polybrominated diphenyl ethers/enzyme-linked immunosorbent assay/indirect competitive inhibition/gas chromatography-mass分类
资源环境引用本文复制引用
刘婧婷,谯华,张彦峰,孙红文,刘洪涛..基于间接竞争抑制ELISA的2,2',4,4'-四溴联苯醚检测方法[J].环境工程学报,2017,11(7):4429-4436,8.基金项目
国家高技术研究发展计划(863计划)项目(2014AA7013037) （863计划）
天津科技兴海项目(KJXH2012-22) （KJXH2012-22）
解放军后勤工程学院院青年科研基金资助项目(YQ15-410307) （YQ15-410307）
