华南农业大学学报2017，Vol.38Issue(4)：12-18,7.DOI:10.7671/j.issn.1001-411X.2017.04.002
印楝素对果蝇厌恶性味觉记忆的诱导及多巴胺能神经元的影响
Induction of aversive taste memory by azadirachtin and its effects on dopaminergic neurons of Drosophila
摘要
Abstract
[Objective] To investigate the inductive effects of azadirachtin on taste memory in Drosophila melanogaster (fruit fly) and the regulation of dopamine signaling in such memory formation.[Method]Aversive taste memory induced by azadirachtin was tested by proboscis extension reflex (PER).Effects of azadirachtin on membrane excitability and receptor currents of dopaminergic neurons in D.melanogaster brain were studied using pressure injection combined with whole-cell patch clamp recording.[Result]Both azadirachtin A and the azadirachtin dry powder could significantly inhibit the probability of PER,which were 60.34% and 17.24% respectively(P<0.007).The effect of dry powder was more obvious.Different clusters of dopaminergic neurons responded variously to azadirachtin.PPL1, PAM and PPM2 subgroups showed increasing trends in excitability, in which the changes of PPL1 cluster neurons were most pronounced.Azadirachtin showed agonistic effect on D1 receptor, and such effect could be inhibited by D1 receptor specific antagonists.[Conclusion]Azadirachtin can induce aversive taste memory in Drosophila, and such memory is regulated by dopaminergic signals in the brain.关键词
印楝素/厌恶性味觉记忆/多巴胺/神经兴奋性/果蝇Key words
azadirachtin/aversive taste memory/dopamine/neuronal excitability/Drosophila分类
农业科技引用本文复制引用
燕颍,顾怀宇,徐汉虹,张志祥..印楝素对果蝇厌恶性味觉记忆的诱导及多巴胺能神经元的影响[J].华南农业大学学报,2017,38(4):12-18,7.基金项目
中国博士后基金(2015M572330) （2015M572330）
国家自然科学基金(31471793) （31471793）
