华南农业大学学报2017，Vol.38Issue(4)：19-24,6.DOI:10.7671/j.issn.1001-411X.2017.04.003
印楝素对小菜蛾胚胎细胞增殖和凋亡的影响
Effects of azadirachtin on proliferation and apoptosis of Plutella xyllostella embryonic cells
摘要
Abstract
[Objective] To investigate the effect and mechanism of azadirachtin on proliferation and apoptosis in Plutella xyllostella embryonic cells.[Method]P.xyllostella embryonic cells were divided into azadirachtin treated and untreated groups, the inhibition rate of cells proliferation was detected using CCK-8 kit.The cell death and apoptotic body were observed using laser confocal microscope after stained by PI and DAPI respectively.The expression of Caspase-3 and the phosphorylation of pathway protein Akt were detected using Western-blotting.[Result]Azadirachtin had significant inhibitory effect on the proliferation of P.xyllostella embryonic cells, and the effect was dose-dependent.IC50 was 4.4 μg·mL-1 in 24 h.Dead cells could be observed in azadirachtin treated group after stained by PI, and apoptotic body could be found in the same group after stained by DAPI.Caspase-3 protein was cleaved and Akt phosphorylation was inhibited.[Conclusion] Azadirachtin has significant inhibitory effect on the proliferation of P.xyllostella embryonic cells.By inhibiting the activation of Akt signaling pathway, azadirachtin can induce cells to produce Caspase-3-dependent type I apoptosis.关键词
印楝素/小菜蛾/胚胎细胞/凋亡/增殖Key words
azadirachtin/Plutella xyllostella/embryonic cell/apoptosis/proliferation分类
农业科技引用本文复制引用
邵雪花,赖多,毛根林,徐汉虹..印楝素对小菜蛾胚胎细胞增殖和凋亡的影响[J].华南农业大学学报,2017,38(4):19-24,6.基金项目
国家自然科学基金(31471793,31601699) （31471793,31601699）
