华南农业大学学报2017，Vol.38Issue(4)：25-29,5.DOI:10.7671/j.issn.1001-411X.2017.04.004
印楝素对min6细胞增殖及胰岛素分泌的影响
Effects of azadirachtin on proliferation and insulin secretion of min6 cells
摘要
Abstract
[Objective] To investigate the effects of azadirachtin on cell proliferation,glucose uptake and insulin secretion in min6 cells and their possible mechanisms. [Method] The min6 cells were cultured in vitro and treated with various concentrations of azadirachtin for 48 h.Cell proliferation was measured.In the presence of 5.5 and 25.0 mmol·L-1 glucose,glucose uptake was measured and insulin secretion in min6 cells were detected by the insulin ELISA kit.[Result] Azadirachtin promoted the proliferation of min6 cells in the presence of 5.5 and 25.0 mmol·L-1 glucose compared to the control group.Compared with the 25.0 mmol·L-1 glucose condition,azadirachtin in the presence of 5.5 mmol·L-1 glucose more significantly promoted glucose uptake and insulin secretion in min6 cells.[Conclusion]Azadirachtin could affect insulin secretion by regulating glucose concentration.关键词
印楝素/min6细胞/细胞增殖/胰岛素分泌/ELISA检测Key words
azadirachtin/min6 cells/cell proliferation/insulin secretion/ELISA test分类
农业科技引用本文复制引用
张玲,王得华,马义,徐汉虹..印楝素对min6细胞增殖及胰岛素分泌的影响[J].华南农业大学学报,2017,38(4):25-29,5.基金项目
广东省自然科学基金(2015A030313333) （2015A030313333）
