华南农业大学学报2017，Vol.38Issue(4)：48-51,4.DOI:10.7671/j.issn.1001-411X.2017.04.008
印楝素干粉的制备及生物活性测定
Preparation and bioactivity determination of azadirachtin-rich dry powder
摘要
Abstract
[Objective] To study the biological activity of azadirachtin-rich dry powder, azadirachtin A and azadirachtin B, and establish a method for determinating azadirachtin content of dry powder.[Method] Azadirachtin-rich dry powder was prepared by ethyl acetate extraction method, the azadirachtin content of dry powder was determined by HPLC and the biological activity was measured by leaf disc test.[Result]AFC50 of zadirachtin-rich dry powder was 0.28 μg·mL-1 against 2nd instar larvae of Spodoptera litura and 70.71 μg·mL-1 against the 3rd instar larvae of Pieris brassicae 24 h after treatment.The antifeedant activity of azadirachtin-rich dry powder was clearly higher than that of azadirachtin A and azadirachtin B.[Conclusion] This preparation method of the dry powder is simple, effective, and has good biological activity.It is recommended for popularization and application.关键词
印楝素干粉/斜纹夜蛾/大菜粉蝶/乙酸乙酯浸提法/叶碟法/HPLC/生物活性Key words
azadirachtin-rich dry powder/Spodoptera litura/Pieris brassicae/ethyl acetate extraction method/leaf disc test/HPLC/bioacitivity分类
农业科技引用本文复制引用
李一鸣,陈孝甜,王勇庆,程东美,张志祥..印楝素干粉的制备及生物活性测定[J].华南农业大学学报,2017,38(4):48-51,4.基金项目
广东省自然科学基金(2016A030313387) （2016A030313387）
