华南农业大学学报2017，Vol.38Issue(4)：52-56,5.DOI:10.7671/j.issn.1001-411X.2017.04.009
腹腔注射亚精胺对小鼠卵巢组织多胺含量及代谢相关基因表达的影响
Effects of intraperitoneal spermidine injection on polyamine content and metabolism-related gene expression in mouse ovary
摘要
Abstract
[Objective] To observe the effects of intraperitoneal injection of spermidine on polyamine metabolism in mouse ovary.[Method] Mice were injected with different doses of spermidine[0(control), 0.05, 0.10 and 0.15 mg·g-1].Real-time fluorescent quantitative PCR was used to determine the ovarian expression levels of key genes in polyamine metabolism.High performance liquid chromatography (HPLC) was used to detect the polyamine levels in mouse ovarian tissues.[Result] After administration of 0.15 mg·g-1 spermidine, the expression of ODC, OAZ1, SPMS, SSAT, PAOX and SMOX genes in ovarian tissues were significantly higher compared to other three groups.SPDS gene expressions of all three spermidine administration groups were significantly lower compared to control.After administration of 0.10 mg·g-1 spermidine, the putrescine and spermidine contents in mouse ovary were significantly higher compared to control, while spermidine administration had no significant effect on spermine content in ovary.[Conclusion] Exogenous spermidine injected intraperitoneally can change polyamine content and polyamine metabolism-related gene expression in mouse ovary.Such effects are dose-dependent.Exogenous spermidine can potentially participate in the regulation of ovarian function through adjusting polyamine metabolism.关键词
亚精胺/鼠/卵巢/多胺代谢/繁殖/基因表达/腹腔注射Key words
spermidine/mouse/ovary/polyamine metabolism/reproduction/gene expression/intraperitoneal injection分类
农业科技引用本文复制引用
陈咨余,姜冬梅,康波,管成,龙诗韵,易治鑫,徐麒麟..腹腔注射亚精胺对小鼠卵巢组织多胺含量及代谢相关基因表达的影响[J].华南农业大学学报,2017,38(4):52-56,5.基金项目
国家自然科学基金(31201798) （31201798）
高等学校博士学科点专项科研基金资助课题(20105103120003) （20105103120003）
