华南农业大学学报2017，Vol.38Issue(4)：69-75,7.DOI:10.7671/j.issn.1001-411X.2017.04.012
中药组方"回乳康"对回乳期奶牛血清E、P4和TGF-β1含量的影响
Effects of Chinese herbal formula "Huirukang" on serum E, P4 and TGF-β1 levels of dairy cows during the milk withdrawal period
摘要
Abstract
[Objective] To explore the impact of Chinese herbal formula "Huirukang" on serum estrogen(E), progesterone(P4) and transforming growth factor β1(TGF-β1) levels in dairy cows during the milk withdrawal period.[Method]Eighty healthy cows in late pregnancy with (15.42±0.71) kg daily milk yield were randomly divided into four groups: control group and Chinese medicine groups(fed 400, 500 and 600 g "Huirukang" daily).The method of gradual milk cessation was used.We collected venous blood from the cow tail on day 0, 1, 3, 5, 7, 9 and 11, and recorded the daily milk yield.ELISA was used to determine the expression levels of serum E, P4 and TGF-β1.[Result]Control group and Chinese medicine groups (400, 500, 600 g) dried off on day 11, 7, 5 and 5 respectively.Serum E levels declined in all four groups, and the decline in control group was slower compared to Chinese medicine groups.P4 level did not change significantly during the milk withdrawal period for control group.P4 levels increased first in Chinese medicine groups, then declined after the milk withdrawal period, and by the end of the test period were still significantly higher compared to control group.TGF-β1 levels increased in all four groups during the milk withdrawal period, and declined after the milk withdrawal period in Chinese medicine groups.The levels of E, TGF-β1 and daily milk yield showed significant correlation between each other during the milk withdrawal period, while P4 had no significant correlation with E, TGF-β1 or daily milk yield.[Conclusion]During the milk withdrawal period, Chinese herbal formula "Huirukang" can significantly promote cows to dry off.E is a negative regulation factor for milk withdrawal, while TGF-β1 is a positive regulation factor.P4 has no significant effect on milk withdrawal.关键词
奶牛/回乳期/中药组方/雌激素/孕酮/转化生长因子β1Key words
cow/milk withdrawal period/Chinese herbal formula/estrogen/progesterone/TGF-β1分类
农业科技引用本文复制引用
巫晓峰,余树民,江涛,肖劲邦,沈留红,姜思汛,钱柏霖,邓俊良,傅宏庆,左之才,曹随忠..中药组方"回乳康"对回乳期奶牛血清E、P4和TGF-β1含量的影响[J].华南农业大学学报,2017,38(4):69-75,7.基金项目
四川省教育厅重点项目(15ZA0024) （15ZA0024）
江苏农牧科技职业学院产业发展关键技术创新项目(NSF201604) （NSF201604）
四川农业大学双支计划项目(03571303) （03571303）
四川农业大学本科科研兴趣培养计划项目(KY2016062) （KY2016062）
