核农学报2017，Vol.31Issue(8)：1494-1499,6.DOI:10.11869/j.issn.100-8551.2017.08.1494
CIDEC的亚细胞定位及其功能初步研究
Localization and Preliminarily Functional Analysis of CIDEC
摘要
Abstract
CIDEC (also known as FSP27) is highly expressed in adipose tissue that promotes intracellular triglyceride (TAG) storage.To explore the mechanism of CIDEC promoting lipid droplet fusion,it had been studied that the diameter and the number of lipid droplets in HepG2 cells incubated with fatty acid were detected,and the result found there were no significant difference before or after treatments.After cells transfected with recombinant vector containing CIDEC was incubated in fatty acid for 24 h,the diameter and number of lipid droplets were measured,its subcellular localization were analyzed by confocal microscopy,and expression of genes related to lipid droplet formation and growth were detected.It indicated that the diameter of lipid droplets increased and the number decreased significantly after overexpression of CIDEC.Moreover,the expressions of PLIN1,CREB1,CREB8 were decreased,while the expressions of CIDEA,CFD were increased.It is speculated that CIDEC may interact with these proteins to cause lipid droplet fusion.This study provides theoretical basis study the mechanism of lipid droplet fusion promoting by CIDEC.关键词
CIDEC/FSP27/脂滴融化/脂肪酸Key words
CIDEC/FSP27/1ipid droplets fusion/fatty acid引用本文复制引用
李红强,李海玉,李婧实,高飞飞,李鑫波,郭振清..CIDEC的亚细胞定位及其功能初步研究[J].核农学报,2017,31(8):1494-1499,6.基金项目
河北省自然科学基金青年基金(C2016407094),河北省高等学校科学研究青年基金(QN2016136) （C2016407094）
