湖南农业大学学报（自然科学版）2017，Vol.43Issue(4)：347-352,6.DOI:10.13331/j.cnki.jhau.2017.04.001
水稻光温敏核不育系育性敏感期高温对异交特性的影响
Effect of high temperature on outcrossing characteristics at the fertile sensitive stage of photo thermo sensitive genic male sterile (PTGMS) rice lines
摘要
Abstract
Four sterile lines (Peiai64S, Y58S, Guangzhan63-2S and H638S) and the restorer line R527 were used as materials. Five temperature gradients(24, 27, 30, 33 and 36℃ in artificial climate chamber) and the natural temperature (as control)were treated to the four sterile lines for 6 days at the fertile sensitive stage of heading to flowering stage respectively, to study the effect of temperature on physiological biochemical characteristics of young spike and outcrossing characteristics. The results showed that the stigma exposure rates of Peiai64S and Y58S were highest at 27℃, which were 86.81% and 86.06% respectively, while the stigma exposure rates of Guangzhan63-2S and H638S were highest at 24℃, they were 76.24% and 81.76%; the stigma vitalities of Peiai64S, Y58S, Guangzhan63-2S, and H638S were best at 24℃, which were 1.96, 2.12, 1.74 and 1.94, respectively; the outcrossing rates of Peiai64S, Guangzhan63-2S and H638S were highest at 24℃, which were 58.87%, 54.22% and 50.50% respectively, while the outcrossing rate of Y58S was highest at 27℃, which was 58.96%; the content of peroxidase (POD) of four sterile lines increased significantly at 33℃ compared with the control, while the content of malondialdehyde (MDA) and proline increased significantly at 36℃ compared with the control. There were differences in temperature sensitivity between the male sterile lines, 24℃ treatment during the sensitive period was the best for Peiai64S fertility-sensitive period, while 27℃ was the best temperature for Y58S, Gangzhan63-2S, H638S.关键词
水稻/光温敏核不育系/育性敏感期/温度/异交特性/生理生化特性Key words
rice/photo and thermo sensitive male sterile line/fertility-sensitive period/temperature/outcrossing characteristics/physiology and biochemistry分类
农业科技引用本文复制引用
王明,刘烨,张海清,刘爱民,肖层林,唐荣,杨永标,蒋凌云,李雪雪..水稻光温敏核不育系育性敏感期高温对异交特性的影响[J].湖南农业大学学报（自然科学版）,2017,43(4):347-352,6.基金项目
国家科技支撑计划项目(2014BAD06B07) （2014BAD06B07）
湖南省财政厅农业专项 （）
湖南省创新项目(XCX15148) （XCX15148）
