湖南农业大学学报（自然科学版）2017，Vol.43Issue(4)：372-376,5.DOI:10.13331/j.cnki.jhau.2017.04.005
芍药查尔酮异构酶基因CHI的表达特性分析
Expression pattern analysis on the chalcone isomerase (CHI) gene inPaeonia lactiflora cultivars
摘要
Abstract
Chalcone isomerase (CHI) is a key gene in regulating the formation of yellow petals. Inner and outer petals from differentPaeonia lactiflora cultivars including Jinhui, Tongyunjinyan, Hongloujinjuat four growing stages (flower-bud stage, initiating bloom stage, bloom stage, wither stage) were selected as materials to study expression pattern and characteristic ofCHI gene. Expression difference ofCHIgene in inner and outer petal at four growing stages among differentPaeonia lactiflora cultivars were conducted using qPCR technology. The results showed that the expression level ofCHI gene exhibited difference in petal tissues from the same cultivar and showed a rise trend from flower bud stage to wither stage; For different cultivars, the expression level ofCHI gene in petal tissues of Tongyunjinyan was apparently higher than that of Jinhuiand Tongyunjinyan at the same growing stages, furthermore, it was apparently higher in inner petals than that in outer petals for the same cultivars at same growing stages. Considering color difference in inner and outer petals at different growing stages and differentPaeonia lactiflora cultivars, it was speculated that the expression level of CHIgene might be related to the color formation of petals inPaeonia lactiflora. The result could provide theoretical guidance for further research on theCHI function and genetic improvement to flower color of Paeonia lactiflora.关键词
芍药/查尔酮异构酶基因CHI/花瓣/基因表达Key words
Paeonia lactiflora/chalcone isomerase (CHI) gene/petal/gene expression分类
生物科学引用本文复制引用
吴彦庆,姜宇,赵大球,陶俊..芍药查尔酮异构酶基因CHI的表达特性分析[J].湖南农业大学学报（自然科学版）,2017,43(4):372-376,5.基金项目
国家自然科学基金(31372097,31400592) （31372097,31400592）
江苏省高校自然科学研究重大项目(13KJA210005) （13KJA210005）
